Species differentiation by internally transcribed spacer PCR and HhaI digestion of fluconazole-resistant Candida krusei, Candida inconspicua, and Candida norvegensis strains

被引：23

作者：

Nho, SG

Anderson, MJ

Moore, CB

Denning, DW

机构：

[1] N MANCHESTER GRP HOSP,DEPT TROP MED & INFECT DIS,MONSALL UNIT,MANCHESTER,LANCS,ENGLAND

[2] HOPE HOSP,DEPT MED,SALFORD M6 8HD,LANCS,ENGLAND

[3] HOPE HOSP,DEPT MICROBIOL,SALFORD M6 8HD,LANCS,ENGLAND

[4] UNIV MANCHESTER,MANCHESTER,LANCS,ENGLAND

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1997年 / 35卷 / 04期

关键词：

D O I：

10.1128/JCM.35.4.1036-1039.1997

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

PCR amplification of the regions containing the internally transcribed spacers and 5.8S rRNA gene of Candida krusei, C. inconspicua, and C. norvegensis yielded fragments of 510, 460, and 500 bp, respectively. HhaI digestion of these fragments yielded species-specific bands. Random amplification of polymorphic DNA with primer R108 showed interspecific discriminatory band patterns. Susceptibilities to fluconazole and amphotericin B were determined.

引用

页码：1036 / 1039

页数：4

共 22 条

[1] USE OF RANDOMLY AMPLIFIED POLYMORPHIC DNA MARKERS TO DISTINGUISH ISOLATES OF ASPERGILLUS-FUMIGATUS [J].