Novel synthetic collagen fibers, poly(PHG), stimulate platelet aggregation through glycoprotein VI

被引：9

作者：

Inoue, Osamu ^{[1
]}

Suzuki-Inoue, Katsue ^{[1
]}

Shinoda, Daisuke ^{[1
]}

Umeda, Yasuto ^{[2
]}

Uchino, Masazumi ^{[2
]}

Takasaki, Sin-ichi ^{[2
]}

Ozaki, Yukio ^{[1
]}

机构：

[1] Univ Yamanashi, Fac Med, Dept Clin & Lab Med, Chuo Ku, Yamanashi 4093898, Japan

[2] Yokohama Res Ctr, Chisso Corp, Kanazawa Ku, Yokohama, Kanagawa 2368605, Japan

来源：

FEBS LETTERS | 2009年 / 583卷 / 01期

关键词：

Collagen; Synthetic collagen; Collagen-related peptide; Blood platelet; Glycoprotein VI; RECEPTOR-GAMMA-CHAIN; TYROSINE PHOSPHORYLATION; ACTIVATION; GPVI; INVOLVEMENT; SYK;

D O I：

10.1016/j.febslet.2008.11.026

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Novel synthetic collagen fibers, poly(PHG) made by polycondensation of Pro-Hyp-Gly, spontaneously assume polymeric structure with molecular weights greater than 10(5). Its application for biomaterials has been explored, but that for a platelet agonist has not been investigated. Poly(PHG)-induced platelet aggregation independently of thromboxane A(2) and integrin alpha 2 beta 1. Poly(PHG)-induced tyrosine phosphorylation of glycoprotein VI (GPVI)-related molecules and failed to activate GPVI/FcR gamma-deficient platelets. Binding of GPVI to poly(PHG) was confirmed by a surface plasmon resonance spectroscopy, suggesting that poly(PHG) activates platelets through GPVI. Poly(PHG) is an useful research tool to investigate GPVI-mediated signals and a substitute for collagen in platelet functional assays. (c) 2008 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：81 / 87

页数：7

共 15 条

[1] Physical and functional association of the Src family kinases Fyn and Lyn with the collagen receptor glycoprotein VI-Fc receptor γ chain complex on human platelets [J].

Ezumi, Y ;

Shindoh, K ;

Tsuji, M ;

Takayama, H .

JOURNAL OF EXPERIMENTAL MEDICINE, 1998, 188 (02) :267-276

[2] Tyrosine phosphorylation of SLP-76 is downstream of Syk following stimulation of the collagen receptor in platelets [J].

Gross, BS ;

Lee, JR ;

Clements, JL ;

Turner, M ;

Tybulewicz, VLJ ;

Findell, PR ;

Koretzky, GA ;

Watson, SP .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (09) :5963-5971

[3] Laminin stimulates spreading of platelets through integrin α6β1-dependent activation of GPVI [J].

Inoue, O ;

Suzuki-Inoue, K ;

McCarty, OJT ;

Moroi, M ;

Ruggeri, ZM ;

Kunicki, TJ ;

Ozaki, Y ;

Watson, SP .

BLOOD, 2006, 107 (04) :1405-1412

[4]

Kehrel B, 1998, BLOOD, V91, P491

[5] Synthesis of poly(Pro-Hyp-Gly)n by direct polycondensation of (Pro-Hyp-Gly)n, where n = 1, 5, and 10, and stability of the triple-helical structure [J].

Kishimoto, T ;

Morihara, Y ;

Osanai, M ;

Ogata, S ;

Kamitakahara, M ;

Ohtsuki, C ;

Tanihara, M .

BIOPOLYMERS, 2005, 79 (03) :163-172

[6] Analysis of the interaction of platelet collagen receptor glycoprotein VI (GPVI)with collagen - A dimeric form of GPVI, but not the monomeric form, shows affinity to fibrous collagen [J].

Miura, Y ;

Takahashi, T ;

Jung, SM ;

Moroi, M .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (48) :46197-46204

[7] INTEGRIN ALPHA-2-BETA-1-INDEPENDENT ACTIVATION OF PLATELETS BY SIMPLE COLLAGEN-LIKE PEPTIDES - COLLAGEN TERTIARY (TRIPLE-HELICAL) AND QUATERNARY (POLYMERIC) STRUCTURES ARE SUFFICIENT ALONE FOR ALPHA-2-BETA-1-INDEPENDENT PLATELET REACTIVITY [J].

MORTON, LF ;

HARGREAVES, PG ;

FARNDALE, RW ;

YOUNG, RD ;

BARNES, MJ .

BIOCHEMICAL JOURNAL, 1995, 306 :337-344

[8] Platelet-collagen interaction: is GPVI the central receptor? [J].

Nieswandt, B ;

Watson, SP .

BLOOD, 2003, 102 (02) :449-461

[9]

Pasquet JM, 1999, MOL CELL BIOL, V19, P8326

[10] Platelet activation and signal transduction by convulxin, a C-type lectin from Crotalus durissus terrificus (tropical rattlesnake) venom via the p62/GPVI collagen receptor [J].

Polgar, J ;

Clemetson, JM ;

Kehrel, BE ;

Wiedemann, M ;

Magnenat, EM ;

Wells, TNC ;

Clemetson, KJ .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (21) :13576-13583

← 1 2 →