Direct inhibitory effect of corticotropin releasing hormone on isolated caecal circular smooth muscle cells of guinea pig via adenylate cyclase system

被引：10

作者：

Iwakiri, Y ^{[1
]}

Chijiiwa, Y ^{[1
]}

Motomura, Y ^{[1
]}

Akiho, H ^{[1
]}

Osame, M ^{[1
]}

Nawata, H ^{[1
]}

机构：

[1] KAGOSHIMA UNIV, FAC MED, DEPT INTERNAL MED 3, KAGOSHIMA 890, JAPAN

来源：

LIFE SCIENCES | 1996年 / 58卷 / 24期

关键词：

corticotropin releasing hormone; cholecystokinin octapeptide; cAMP; isolated smooth muscle cells; caecum;

D O I：

10.1016/0024-3205(96)00219-6

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Smooth muscle cells isolated separately from the caecal circular smooth muscle layer of the guinea pig were used to investigate whether corticotropin releasing hormone (CRH) can inhibit directly the contraction produced by cholecystokinin octapeptide (CCK-8). In addition, the role of adenylate cyclase and guanylate cyclase in the direct inhibitory effect of CRH was examined. CRH inhibited the contractile response produced by 10(-9) M CCK-8 in a concentration-dependent manner, with an IC50 value of 0.16 nM. An inhibitor of particulate guanylate cyclase and an inhibitor of soluble guanylate cyclase had no significant effect of the relaxation produced by CRH. In contrast, an inhibitor of adenylate cyclase and an inhibitor of cAMP-dependent protein kinase significantly inhibited the relaxation produced by CRH. This is the first report demonstrating the direct inhibitory action of CRH on the isolated caecal smooth muscle cells via adenylate cyclase system.

引用

页码：2243 / 2249

页数：7

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