Polysaccharides from Gracilaria corticata: Sulfation, chemical characterization and anti-HSV activities

被引：67

作者：

Chattopadhyay, Kausik ^{[1
]}

Ghosh, Tuhin ^{[1
]}

Pujol, Carlos A. ^{[2
]}

Carlucci, Maria J. ^{[2
]}

Damonte, Elsa B. ^{[2
]}

Ray, Bimalendu ^{[1
]}

机构：

[1] Univ Burdwan, Dept Chem, Nat Prod Lab, Burdwan 713104, W Bengal, India

[2] Univ Buenos Aires, Fac Ciencias Exactas & Nat, Virol Lab, Dept Quim Biol, RA-1428 Buenos Aires, DF, Argentina

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2008年 / 43卷 / 04期

关键词：

Gracilaria corticata; Galactan sulfate; Anti-HSV activity;

D O I：

10.1016/j.ijbiomac.2008.07.009

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

In this study, we have analyzed water-extracted polysaccharides of Gracilaria corticata. The water extract (WE), a galactan-containing sub-fraction (F3) and their hyper sulfated derivatives (WES1, WES2, F3S1 and F3S2) had anti-HSV activity with inhibitory concentration 50% (IC50) from 1.1 to 27.4 mu g/ml. Sub-fraction F3, which has a molecular mass of 30 kDa, consists of a backbone of beta-(1 -> 3) and alpha-(1 -> 4)-linked-galactopyranosyl residues. This linear galactan contained Gal(2)Xyl(1), Gal(2)AnGal(2), Gal(4) and Me-Gal(3)AnGal(2) as oligomeric building subunits. Sulfate group was located at C-4 of (1 -> 3)-linked galactopyranosyl residues of the native galactan, and appeared to be very important for the anti-herpetic activity. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：346 / 351

页数：6

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