The D1-A2 and D2-A2 pairs of splice sites from human immunodeficiency virus type 1 are highly efficient in vitro, in spite of an unusual branch site

被引:21
作者
Damier, L [1 ]
Domenjoud, L [1 ]
Branlant, C [1 ]
机构
[1] UNIV NANCY 1, LAB ENZYMOL & GENIE GENET, URA CNRS 457, F-54506 VANDOEUVRE LES NANCY, FRANCE
关键词
D O I
10.1006/bbrc.1997.7091
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Using in vitro splicing assays with HeLa cell nuclear extracts, we showed that the HIV-1 pairs of splice sites D1-A2 and D2-A2 are efficiently used in vitro, as compared to the control D1-A2 pair of sites from the E3 transcription unit of human adenovirus-2. The strong efficiency of the two HIV-1 pairs of sites is surprising, as we also showed by primer extension analysis that the branch-site sequence used at the HIV-1 acceptor site A2 is UAGCAGA, with a dominant utilization of the ultimate G as the branched residue. No significant increase of the splicing efficiency was observed upon replacement of the wild-type branch-site sequence by a canonical sequence, in spite of the utilization of an a residue as the branched nucleotide. Results are discussed taking into account the present knowledge on branch-site selection. (C) 1997 Academic Press.
引用
收藏
页码:182 / 187
页数:6
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