A method to size DNA fragments from 50 to 800 bp on a DNA analyser

被引：6

作者：

Avis, PG

Feldheim, KA

机构：

[1] Field Museum, Dept Bot, Chicago, IL 60605 USA

[2] Field Museum, Pritzker Mol Lab, Chicago, IL 60605 USA

来源：

MOLECULAR ECOLOGY NOTES | 2005年 / 5卷 / 04期

关键词：

capillary electrophoresis; fluorescently labelled size standard; fragment analysis;

D O I：

10.1111/j.1471-8286.2005.01105.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We present a method to size DNA fragments from 50 to 800 bp on a DNA analyser using an alternative size standard (X-Rhodamine MapMarker 1000) run on a capillary electrophoresis system (Applied Biosystem's 3730 DNA Analyser). This method avoids several limitations imposed by manufacturer-developed size standards, instruments and software and offers a more robust approach to conducting DNA size fragment analyses.

引用

页码：969 / 970

页数：2

共 8 条

[1]

Delmotte F, 2001, BIOTECHNIQUES, V31, P810

[2] Universal method for producing ROX-labeled size standards suitable for automated genotyping [J].

DeWoody, JA ;

Schupp, J ;

Kenefic, L ;

Busch, J ;

Murfitt, L ;

Keim, P .

BIOTECHNIQUES, 2004, 37 (03) :348-+

[3] Vertical niche differentiation of ectomycorrhizal hyphae in soil as shown by T-RFLP analysis [J].

Dickie, IA ;

Xu, B ;

Koide, RT .

NEW PHYTOLOGIST, 2002, 156 (03) :527-535

[4]

Gao QF, 1999, ELECTROPHORESIS, V20, P1518, DOI 10.1002/(SICI)1522-2683(19990601)20:7<1518::AID-ELPS1518>3.3.CO

[5]

2-X

[6]

Gill P, 2001, ELECTROPHORESIS, V22, P2670, DOI 10.1002/1522-2683(200108)22:13<2670::AID-ELPS2670>3.0.CO

[7]

2-1

[8] A simple and inexpensive method for producing fluorescently labelled size standard [J].

Symonds, VV ;

Lloyd, AM .

MOLECULAR ECOLOGY NOTES, 2004, 4 (04) :768-771

← 1 →