Stable expression of FRET biosensors: A new light in cancer research

被引：86

作者：

Aoki, Kazuhiro ^{[1
]}

Komatsu, Naoki ^{[1
]}

Hirata, Eishu ^{[1
]}

Kamioka, Yuji ^{[2
,3
]}

Matsuda, Michiyuki ^{[1
,2
]}

机构：

[1] Kyoto Univ, Lab Bioimaging & Cell Signaling, Grad Sch Biostudies, Kyoto, Japan

[2] Kyoto Univ, Grad Sch Med, Dept Pathol & Biol Dis, Kyoto, Japan

[3] Kyoto Univ, Innovat Technohub Integrated Med Bioimaging, Kyoto, Japan

来源：

CANCER SCIENCE | 2012年 / 103卷 / 04期

基金：

日本学术振兴会;

关键词：

RESONANCE ENERGY-TRANSFER; CYAN FLUORESCENT PROTEIN; LIVING CELLS; DYNAMIC-RANGE; RHO-GTPASES; INDICATORS; VISUALIZATION; ACTIVATION; KINASE; BRIGHT;

D O I：

10.1111/j.1349-7006.2011.02196.x

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 [肿瘤学];

摘要：

The constituents of the oncogene signal transduction pathway are promising targets for anticancer drugs. Despite the wealth of available knowledge regarding their molecular properties, the spatiotemporal regulation of the signaling molecules remains elusive. Biosensors based on the principle of FRET have been developed to visualize the activities of the signaling molecules in living cells. However, difficulties in the development of sensitive FRET biosensors have prevented their widespread use in cancer research. The lack of cell lines constitutively expressing a FRET biosensor has also limited their use. In this review, we will introduce the principle of FRET-based biosensors, describe an optimized backbone of the FRET biosensors, techniques to express FRET biosensors stably in the cells, and discuss the future perspectives of FRET biosensors in cancer research. (Cancer Sci 2012; 103: 614619)

引用

页码：614 / 619

页数：6

共 32 条

[1]

Directed evolution of a monomeric, bright and photostable version of Clavularia cyan fluorescent protein:: structural characterization and applications in fluorescence imaging [J].