HSP27 phosphorylation is correlated with ADP-induced platelet granule secretion

被引：33

作者：

Kato, Hisaaki ^{[1
,2
]}

Takai, Shinji ^{[1
]}

Matsushima-Nishiwaki, Rie ^{[1
]}

Adachi, Seiji ^{[1
]}

Minamitani, Chiho ^{[4
]}

Otsuka, Takanobu ^{[4
]}

Tokuda, Haruhiko ^{[1
,3
]}

Akamatsu, Shigeru ^{[5
]}

Doi, Tomoaki ^{[1
,2
]}

Ogura, Shinji ^{[2
]}

Kozawa, Osamu ^{[1
]}

机构：

[1] Gifu Univ, Grad Sch Med, Dept Pharmacol, Gifu 5011194, Japan

[2] Gifu Univ, Grad Sch Med, Dept Emergency & Disaster Med, Gifu 5011194, Japan

[3] Natl Hosp Geriatr Med, Natl Ctr Geriatr & Gerontol, Dept Clin Lab, Aichi, Japan

[4] Nagoya City Univ, Grad Sch Med Sci, Dept Orthoped Surg, Aichi, Japan

[5] Matsunami Gen Hosp, Dept Intens Care Med, Gifu, Japan

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 2008年 / 475卷 / 01期

关键词：

HSP27; platelet; phosphorylation; ADP; granule secretion;

D O I：

10.1016/j.abb.2008.04.023

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Adenosine diphosphate (ADP) plays a crucial role in hemostasis and thrombosis by activating platelets. ADP has been reported to induce heat-shock protein (HSP) 27 phosphorylation in human platelets. However, the exact role of HSP27 phosphorylation in human platelets has not yet been clarified. In the present study, we investigated the mechanisms and the roles of ADP-induced HSP27 phosphorylation in human platelets. We showed for the first time that both of decreased phosphorylation levels of HSP27 by PD98059, a MEK1/2 inhibitor and SB203580, a p38 MAPK inhibitor were correlated with the suppressed levels of platelet granule secretion but not with platelet aggregation. Furthermore, the inhibition of either the p44/p42 MAPK or p38 MAPK pathways had no effect on ADP-induced platelet aggregation. These results strongly suggest that the ADP-induced phosphorylation of HSP27 via p44/p42 MAPK and/or p38 MAPK is therefore sufficient for platelet granule secretion but not for platelet aggregation in humans. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：80 / 86

页数：7

共 33 条

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