Immobilized metal ion affinity chromatography: a review on its applications

被引：231

作者：

Cheung, Randy Chi Fai ^{[1
]}

Wong, Jack Ho ^{[1
]}

Ng, Tzi Bun ^{[1
]}

机构：

[1] Chinese Univ Hong Kong, Fac Med, Sch Biomed Sci, Hong Kong, Hong Kong, Peoples R China

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2012年 / 96卷 / 06期

关键词：

Immobilized metal ion affinity chromatography; Proteins; Purification; Review; HISTIDINE-TAGGED PROTEIN; CHAIN FV ANTIBODIES; PURITY FACTOR-IX; RECOMBINANT PROTEINS; CAPILLARY ELECTROPHORESIS; HUMAN PROLACTIN; PURIFICATION; TAGS; EXPRESSION; IMAC;

D O I：

10.1007/s00253-012-4507-0

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 090105 [作物生产系统与生态工程];

摘要：

After 35 years of development, immobilized metal ion affinity chromatography (IMAC) has evolved into a popular protein purification technique. This review starts with a discussion of its mechanism and advantages. It continues with its applications which include the purification of histidine-tagged proteins, natural metal-binding proteins, and antibodies. IMAC used in conjunction with mass spectroscopy for phosphoprotein fractionation and proteomics is also covered. Finally, this review addresses the developments, limitations, and considerations of IMAC in the biopharmaceutical industry.

引用

页码：1411 / 1420

页数：10

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