Measurement of small dense low-density lipoprotein particles

被引:119
作者
Hirano, T
Ito, Y
Yoshino, G
机构
[1] Showa Univ, Sch Med, Dept Internal Med 2, Div Diabet & Metab,Shinagawa Ku, Tokyo 1428666, Japan
[2] Dept Res & Dev, Tokyo, Japan
[3] Toho Univ, Sch Med, Dept Diabet Metab & Endocrinol, Tokyo, Japan
关键词
small dense LDL; cholesterol; apolipoprotein B; precipitation;
D O I
10.5551/jat.12.67
中图分类号
R6 [外科学];
学科分类号
1002 [临床医学]; 100210 [外科学];
摘要
Low density lipoprotein (LDL) particles are heterogenous with respect to their size, density and lipid composition. Among LDL particles, the smaller and denser LDL particles [small dense (sd) LDL] are more atherogenic and the sd LDL phenotype is strongly associated with development of coronary heart disease. Here we will review various methods for measurement of scl LDL. Although ultracentrifugation, nuclear magnetic resonance (NMR) spectroscopy and gradient-gel electrophoresis (GGE) are usually employed for the measurement of sd LDL, such methods are either too laborious or expensive for general clinical use. We recently established a simple precipitation method for the quantification of sd LDL. This method is applicable to routine clinical use and allows the rapid measurement of a large number of samples.
引用
收藏
页码:67 / 72
页数:6
相关论文
共 24 条
[1]
PROSPECTIVE-STUDY OF SMALL LDLS AS A RISK FACTOR FOR NON-INSULIN-DEPENDENT DIABETES-MELLITUS IN ELDERLY MEN AND WOMEN [J].
AUSTIN, MA ;
MYKKANEN, L ;
KUUSISTO, J ;
EDWARDS, KL ;
NELSON, C ;
HAFFNER, SM ;
PYORALA, K ;
LAAKSO, M .
CIRCULATION, 1995, 92 (07) :1770-1778
[2]
AUSTIN MA, 1988, JAMA-J AM MED ASSOC, V260, P1917
[3]
ATHEROGENIC LIPOPROTEIN PHENOTYPE - A PROPOSED GENETIC-MARKER FOR CORONARY HEART-DISEASE RISK [J].
AUSTIN, MA ;
KING, MC ;
VRANIZAN, KM ;
KRAUSS, RM .
CIRCULATION, 1990, 82 (02) :495-506
[4]
Austin Melissa A., 1994, Current Opinion in Lipidology, V5, P395, DOI 10.1097/00041433-199412000-00002
[5]
Metabolic origins and clinical significance of LDL heterogeneity [J].
Berneis, KK ;
Krauss, RM .
JOURNAL OF LIPID RESEARCH, 2002, 43 (09) :1363-1379
[6]
Accumulation of lipoprotein fractions and subfractions in the arterial wall, determined in an in vitro perfusion system [J].
Bjornheden, T ;
Babyi, A ;
Bondjers, G ;
Wiklund, O .
ATHEROSCLEROSIS, 1996, 123 (1-2) :43-56
[7]
Compositional differences of LDL particles in normal subjects with LDL subclass phenotype A and LDL subclass phenotype B [J].
Capell, WH ;
Zambon, A ;
Austin, MA ;
Brunzell, JD ;
Hokanson, JE .
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 1996, 16 (08) :1040-1046
[8]
Chapman MJ, 1998, EUR HEART J, V19, pA24
[9]
Rapid separation of LDL subclasses by iodixanol gradient ultracentrifugation [J].
Davies, IG ;
Graham, JM ;
Griffin, BA .
CLINICAL CHEMISTRY, 2003, 49 (11) :1865-1872
[10]
ROLE OF PLASMA TRIGLYCERIDE IN THE REGULATION OF PLASMA LOW-DENSITY-LIPOPROTEIN (LDL) SUBFRACTIONS - RELATIVE CONTRIBUTION OF SMALL, DENSE LDL TO CORONARY HEART-DISEASE RISK [J].
GRIFFIN, BA ;
FREEMAN, DJ ;
TAIT, GW ;
THOMSON, J ;
CASLAKE, MJ ;
PACKARD, CJ ;
SHEPHERD, J .
ATHEROSCLEROSIS, 1994, 106 (02) :241-253