Insoluble and soluble immune complexes activate neutrophils by distinct activation mechanisms: changes in functional responses induced by priming with cytokines

Background: Rheumatoid synovial fluid contains both soluble and insoluble, immune complexes that can activate infiltrating immune cells such as neutrophils. Objectives: To determine if these different complexes activate neutrophils through similar or different receptor signalling pathways. In particular, to determine the circumstances which result in the secretion of tissue damaging reactive oxygen metabolites and granule enzymes. Methods: Blood neutrophils were incubated with synthetic soluble and insoluble immune complexes and the ability to generate reactive oxidants tested by luminescence or spectrophotometric assays that distinguished between intracellular and extracellular production. Degranulation of myeloperoxidase and lactoferrin was determined by western blotting. The roles of Fc gamma RII (CD32) and Fc gamma RIIIb (CD16) were determined by incubation with Fab/F(ab ')(2) fragments before activation. The effect of cytokine priming was determined by incubation with GM-CSF. Results: Insoluble immune complexes activated unprimed, neutrophils, but most of the oxidants produced were intracellular. This activation required Fc gamma RIIIb, but not Fc gamma RII function. Soluble complexes failed to activate unprimed neutrophils but generated a rapid and extensive secretion of reactive oxygen metabolites when the cells were primed with granulocyte-macrophage colony stimulating factor (GM-CSF). This activity required both Fc gamma RII and Fc gamma RIIIb function. Insoluble immune complexes activated the release of granule enzymes from primed or unprimed neutrophils, but the kinetics of release did not parallel those of secretion of reactive oxygen metabolites. Only primed neutrophils released enzymes in response to soluble complexes. Conclusions: Soluble and insoluble immune complexes activate neutrophils by separate receptor signalling pathways. Profound changes in neutrophil responsiveness to these complexes occur after cytokine priming.