A Homogeneous Chemiluminescent Immunoassay Method

被引：67

作者：

Akhavan-Tafti, Hashem ^{[1
]}

Binger, Dean G. ^{[2
]}

Blackwood, John J. ^{[2
]}

Chen, Ying ^{[2
]}

Creager, Richard S. ^{[2
]}

de Silva, Renuka ^{[1
]}

Eickholt, Robert A. ^{[1
]}

Gaibor, Jose E. ^{[2
]}

Handley, Richard S. ^{[1
]}

Kapsner, Kenneth P. ^{[2
]}

Lopac, Senja K. ^{[2
]}

Mazelis, Michael E. ^{[1
]}

McLernon, Terri L. ^{[2
]}

Mendoza, James D. ^{[2
]}

Odegaard, Bruce H. ^{[2
]}

Reddy, Sarada G. ^{[1
]}

Salvati, Michael ^{[2
]}

Schoenfelner, Barry A. ^{[1
]}

Shapir, Nir ^{[2
]}

Shelly, Katherine R. ^{[2
]}

Todtleben, Jeff C. ^{[2
]}

Wang, Guoping ^{[1
]}

Xie, Wenhua ^{[1
]}

机构：

[1] Beckman Coulter Inc, Southfield, MI 48033 USA

[2] Beckman Coulter Inc, Chaska, MN 55318 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2013年 / 135卷 / 11期

关键词：

ENZYME FRAGMENT COMPLEMENTATION; FLUORESCENCE POLARIZATION ASSAYS; LINKED-IMMUNOSORBENT-ASSAY; HIGH-THROUGHPUT; BINDING-KINETICS; KINASE;

D O I：

10.1021/ja312039k

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A new homogeneous chemiluminescent immunoassay method featuring the use of specific binding members separately labeled with an acridan-based chemiluminescent compound and a peroxidase is reported. Formation of an immunocomplex brings the chemiluminescent compound and the peroxidase into close proximity. Without any separation steps, a chemiluminescent signal is generated upon addition of a trigger solution, and the intensity is directly correlated to the quantity of the analyte.

引用

页码：4191 / 4194

页数：4

共 39 条

[1]

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[2]

Akhavan-Tafti H., 2007, U.S. Patent, Patent No. [7,247,726, 7247726]

[3]

Akhavan-Tafti H., 2004, IVD TECHNOL, V10, P33

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