Quantitative biomarkers of human skin photoaging based on intrinsic second harmonic generation signal

被引：7

作者：

Zhuo, Shuangmu ^{[1
]}

Zhu, Xiaoqin ^{[1
]}

Chen, Jianxin ^{[1
]}

Xie, Shusen ^{[1
]}

机构：

[1] Fujian Normal Univ, Inst Laser & Optoelect Technol, Key Lab OptoElect Sci & Technol Med, Fujian Prov Key Lab Photon Technol,Minist Educ, Fuzhou 350007, Peoples R China

来源：

SCANNING | 2013年 / 35卷 / 04期

基金：

中国国家自然科学基金;

关键词：

collagen; human skin photoaging; second harmonic generation microscopy; COLLAGEN; DERMIS;

D O I：

10.1002/sca.21062

中图分类号：

TH7 [仪器、仪表];

学科分类号：

080401 [精密仪器及机械];

摘要：

Collagen change is a major feature in the photoaged human skin. Here, we present the use of intrinsic second harmonic generation (SHG) signal as a novel means to quantify collagen change with photoaging. We obtain the SHG images of the superficial dermis from ex vivo the cheek skin and the abdomen skin of eight patients aged 55-60 years. The results show that SHG signal can quantitatively reveal collagen change between normal and photoaged human skin in three dimensions. By comparing normal with photoaged dermis, there are significant differences in the collagen content and fine structure, providing substantial potential to be applied in vivo for the clinical diagnosis of human skin photoaging. SCANNING 35: 273-276, 2013. (c) 2012 Wiley Periodicals, Inc.

引用

收藏

页码：273 / 276

页数：4

相关论文

共 14 条

[1]

Dynamic imaging of collagen and its modulation in tumors in vivo using second-harmonic generation [J].

Brown, E ;

McKee, T ;

diTomaso, E ;

Pluen, A ;

Seed, B ;

Boucher, Y ;

Jain, RK .

NATURE MEDICINE, 2003, 9 (06) :796-800

[2]

Second harmonic generation microscopy: principles and applications to disease diagnosis [J].

Campagnola, Paul J. ;

Dong, Chen-Yuan .

LASER & PHOTONICS REVIEWS, 2011, 5 (01) :13-26

[3]

Scoring of collagen organization in healthy and diseased human dermis by multiphoton microscopy [J].

Cicchi, Riccardo ;

Kapsokalyvas, Dimitrios ;

De Giorgi, Vincenzo ;

Maio, Vincenza ;

Van Wiechen, Annelies ;

Massi, Daniela ;

Lotti, Torello ;

Pavone, Francesco S. .

JOURNAL OF BIOPHOTONICS, 2010, 3 (1-2) :34-43

[4]

RESTORATION OF COLLAGEN FORMATION IN PHOTODAMAGED HUMAN SKIN BY TRETINOIN (RETINOIC ACID) [J].

GRIFFITHS, CEM ;

RUSSMAN, AN ;

MAJMUDAR, G ;

SINGER, RS ;

HAMILTON, TA ;

VOORHEES, JJ .

NEW ENGLAND JOURNAL OF MEDICINE, 1993, 329 (08) :530-535

[5]

In vivo assessment of human skin aging by multiphoton laser scanning tomography [J].

Koehler, Martin Johannes ;

Koenig, Karsten ;

Elsner, Peter ;

Bueckle, Rainer ;

Kaatz, Martin .

OPTICS LETTERS, 2006, 31 (19) :2879-2881

[6]

Discrimination of basal cell carcinoma from normal dermal stroma by quantitative multiphoton imaging [J].

Lin, Sung-Jan ;

Jee, Shiou-Hwa ;

Kuo, Chien-Jui ;

Wu, Ruei-Jr ;

Lin, Wei-Chou ;

Chen, Jau-Shiuh ;

Liao, Yi-Hua ;

Hsu, Chih-Jung ;

Tsai, Tsen-Fang ;

Chen, Yang-Fang ;

Dong, Chen-Yuan .

OPTICS LETTERS, 2006, 31 (18) :2756-2758

[7]

In vivo three-dimensional birefringence analysis shows collagen differences between young and old photo-aged human skin [J].

Sakai, Shingo ;

Yamanari, Masahiro ;

Miyazawa, Arata ;

Matsumoto, Masayuki ;

Nakagawa, Noriaki ;

Sugawara, Tomoko ;

Kawabata, Keigo ;

Yatagai, Toyohiko ;

Yasuno, Yoshiaki .

JOURNAL OF INVESTIGATIVE DERMATOLOGY, 2008, 128 (07) :1641-1647

[8]

Differentiation of normal and cancerous lung tissues by multiphoton imaging [J].

Wang, Chun-Chin ;

Li, Feng-Chieh ;

Wu, Ruei-Jhih ;

Hovhannisyan, Vladimir A. ;

Lin, Wei-Chou ;

Lin, Sung-Jan ;

So, Peter T. C. ;

Dong, Chen-Yuan .

JOURNAL OF BIOMEDICAL OPTICS, 2009, 14 (04)

[9]

Multimode nonlinear optical imaging of the dermis in ex vivo human skin based on the combination of multichannel mode and Lambda mode [J].

Zhuo, Shuangmu ;

Chen, Jianxin ;

Luo, Tianshu ;

Zou, Dingsong ;

Zhao, Jingjun .

OPTICS EXPRESS, 2006, 14 (17) :7810-7820

[10]

Quantitative biomarkers of colonic dysplasia based on intrinsic second-harmonic generation signal [J].

Zhuo, Shuangmu ;

Zhu, Xiaoqin ;

Wu, Guizhu ;

Chen, Jianxin ;

Xie, Shusen .

JOURNAL OF BIOMEDICAL OPTICS, 2011, 16 (12)