Skeletal muscle fibers express major histocompatibility complex class II antigens independently of inflammatory infiltrates in inflammatory myopathies

被引:121
作者
Englund, P
Lindroos, E
Nennesmo, I
Klareskog, L
Lundberg, IE
机构
[1] Karolinska Inst, Dept Med, Stockholm, Sweden
[2] Karolinska Inst, Rheumatol Unit, Stockholm, Sweden
[3] Karolinska Inst, Dept Microbiol, Stockholm, Sweden
[4] Karolinska Inst, Dept Pathol, Stockholm, Sweden
[5] Karolinska Inst, Dept Immunol, Stockholm, Sweden
[6] Huddinge Univ Hosp, Div Pathol, Huddinge, Sweden
关键词
D O I
10.1016/S0002-9440(10)62513-8
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
The aim of our study was to address the question of whether muscle fibers express major histocompatibility complex (MHC) class H in inflammatory myopathies. For this purpose we performed a systematic study of MHC class H antigen expression on muscle fiber membranes in muscle tissue from polymyositis and dermatomyositis patients in various stages of disease activity. Thirty-two patients with classical clinical signs of myositis were divided into subgroups depending on duration of clinical signs of myositis and presence or absence of inflammatory infiltrates in muscle tissue. Immunohistochemistry as well as double-immunofluorescence stainings were used to identify the presence of MHC class H in muscle tissue. MHC class I was included for comparison. Quantification of positive staining was performed using an image analysis system in addition to evaluation by manual microscopic scoring and laser confocal microscopy. It was demonstrated that a significant proportion of skeletal muscle fibers in inflammatory myopathies express MHC class II as well as MHC class I and that MHC antigen expression is independent of the inflammatory cell infiltration. Furthermore, there were no differences in staining pattern between polymyositis and dermatomyrositis patients. Our results indicate that MHC class II and MHC class I molecules may be involved in initiating and maintaining the pathological condition in myositis rather than only being a consequence of a preceding local inflammation.
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页码:1263 / 1273
页数:11
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