Surface modification of porous graphite for ion exchange chromatography

被引:35
作者
Knox, JH
Wan, QH
机构
[1] Department of Chemistry, University of Edinburgh, Edinburgh EH9 3JJ, West Mains Road
关键词
ion exchange chromatography; porous graphite; adsorptive stationary phases;
D O I
10.1007/BF02271060
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Three distinct types of weak anion exchanger have been prepared by adsorption of polyethyleneimine (PEI) on to porous graphite (PGC): PGC coated dynamically with PEI, PGC coated with. an insoluble monolayer of PEI, and PGC coated with a cross-linked polymeric PEI. Using model solutes iodate, bromide, nitrate and nitrite, dissolved in aqueous buffers, the modified PGC's show typical ion exchange behaviour, and give chromatographic performance similar to that of bonded ion-exchange silica gels. Retention is reduced by increasing the ionic strength of the buffer, and by increasing the pH. A mechanism to explain the observed trends is presented.
引用
收藏
页码:83 / 88
页数:6
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