Gene expression pattern in hepatic stem/progenitor cells during rat fetal development using complementary DNA microarrays

被引:46
作者
Petkov, PM
Zavadil, J
Goetz, D
Chu, T
Carver, R
Rogler, CE
Bottinger, EP
Shafritz, DA
Dabeva, MD
机构
[1] Albert Einstein Coll Med, Marion Bessin Liver Res Ctr, Bronx, NY 10461 USA
[2] Albert Einstein Coll Med, Dept Med, Bronx, NY 10461 USA
[3] Albert Einstein Coll Med, Ctr Biotechnol, Bronx, NY 10461 USA
[4] Albert Einstein Coll Med, Div Nephrol, Bronx, NY 10461 USA
关键词
D O I
10.1002/hep.20088
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
To identify new and differentially expressed genes in rat fetal liver epithelial stem/progenitor cells during their proliferation, lineage commitment, and differentiation, we used a high throughput method-mouse complementary DNA (cDNA) microarrays-for analysis of gene expression. The gene expression pattern of rat hepatic cells was studied during their differentiation in vivo: from embryonic day (ED) 13 until adulthood. The differentially regulated genes were grouped into two dusters: a duster of up-regulated genes comprised of 281 clones and a cluster of down-regulated genes comprised of 230 members. The expression of the latter increased abruptly between ED 16 and ED 17. Many of the overexpressed genes from the first duster fall into distinct, differentially expressed functional groups: genes related to development, morphogenesis, and differentiation; calcium- and phospholipid-binding proteins and signal transducers; and cell adhesion, migration, and matrix proteins. Several other functional groups of genes that are initially down-regulated, then increase during development, also emerged. genes related to inflammation, blood coagulation, detoxification, serum proteins, amino acids, lipids, and carbohydrate metabolism. Twenty-eight genes overexpressed in fetal liver that were not detected in adult liver are suggested as potential markers for identification of liver progenitor cells. In conclusion, our data show that the gene expression program of fetal hepatoblasts differs profoundly from that of adult hepatocytes and that it is regulated in a specific manner with a major switch at ED 16 to 17, marking a dramatic change in the gene expression program during the transition of fetal liver progenitor cells from an undifferentiated to a differentiated state.
引用
收藏
页码:617 / 627
页数:11
相关论文
共 47 条
[1]   Pluripotential liver stem cells: Facultative stem cells located in the biliary tree [J].
Alison, MR ;
Golding, MHC ;
Sarraf, CE .
CELL PROLIFERATION, 1996, 29 (07) :373-402
[2]   Regulation of cell apoptosis by protein kinase c δ [J].
Brodie, C ;
Blumberg, PM .
APOPTOSIS, 2003, 8 (01) :19-27
[3]  
Cano-Gauci DF, 1999, J CELL BIOL, V146, P255
[4]   Making and reading microarrays [J].
Cheung, VG ;
Morley, M ;
Aguilar, F ;
Massimi, A ;
Kucherlapati, R ;
Childs, G .
NATURE GENETICS, 1999, 21 (Suppl 1) :15-19
[5]   A gene encoding an intestinal-enriched member of the Kruppel-like factor family expressed in intestinal epithelial cells [J].
Conkright, MD ;
Wani, MA ;
Anderson, KP ;
Lingrel, JB .
NUCLEIC ACIDS RESEARCH, 1999, 27 (05) :1263-1270
[6]   Proliferation and differentiation of fetal liver epithelial progenitor cells after transplantation into adult rat liver [J].
Dabeva, MD ;
Petkov, PM ;
Sandhu, J ;
Oren, R ;
Laconi, E ;
Hurston, E ;
Shafritz, DA .
AMERICAN JOURNAL OF PATHOLOGY, 2000, 156 (06) :2017-2031
[7]   NEUROPEPTIDE-Y - LOCALIZATION IN THE CENTRAL-NERVOUS-SYSTEM AND NEUROENDOCRINE FUNCTIONS [J].
DANGER, JM ;
TONON, MC ;
JENKS, BG ;
SAINTPIERRE, S ;
MARTEL, JC ;
FASOLO, A ;
BRETON, B ;
QUIRION, R ;
PELLETIER, G ;
VAUDRY, H .
FUNDAMENTAL & CLINICAL PHARMACOLOGY, 1990, 4 (03) :307-340
[8]   Peg3/Pw1 promotes p53-mediated apoptosis by inducing Bax translocation from cytosol to mitochondria [J].
Deng, YB ;
Wu, XW .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (22) :12050-12055
[9]  
Dubois AM., 1963, The Liver, P1, DOI [10.1016/B978-1-4832-2824-2.50007-8, DOI 10.1016/B978-1-4832-2824-2.50007-8]
[10]   The role of hepatocytes and oval cells in liver regeneration and repopulation [J].
Fausto, N ;
Campbell, JS .
MECHANISMS OF DEVELOPMENT, 2003, 120 (01) :117-130