Regulation of endoreduplication in maize (Zea mays L.) endosperm.: Isolation of a novel B1-type cyclin and its quantitative analysis

被引：36

作者：

Sun, YJ ^{[1
]}

Flannigan, BA ^{[1
]}

Setter, TL ^{[1
]}

机构：

[1] Cornell Univ, Dept Soil Crop & Atmospher Sci, Ithaca, NY 14853 USA

来源：

PLANT MOLECULAR BIOLOGY | 1999年 / 41卷 / 02期

关键词：

cell cycle; cyclin B; flow cytometry; quantitative RT-PCR;

D O I：

10.1023/A:1006315625486

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To investigate the involvement of cyclin in mitotic and endoreduplicative cell cycle control, we have isolated a mitotic cyclin clone from a maize endosperm cDNA library. The deduced amino acid sequence of this clone identifies a novel B1-type cyclin with distinctly different sequence in regions with putative involvement in intracellular localization. This cyclin, designated Zeama;CycB1;3 (CycZme1), was shown by RNA gel blots and quantitative RT-PCR to be specific for tissues engaging in cell proliferation. It accumulated in metaphase-arrested cells and declined rapidly upon release into G1 phase. During the transition from mitosis to endoreduplication in maize endosperm, CycZme1 transcript declined precipitously while transcripts associated with S phase (histone-H3 and PCNA) and multiple phases of the cell cycle (Cdc2, alpha-tubulin) remained at moderate to high levels. We conclude that CycZme1 down-regulation is involved in the cellular transition to endoreduplication.

引用

页码：245 / 258

页数：14

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