Arabidopsis MAP kinase 4 regulates gene expression through transcription factor release in the nucleus

被引:376
作者
Qiu, Jin-Long [1 ]
Fiil, Berthe Katrine [1 ]
Petersen, Klaus [1 ]
Nielsen, Henrik Bjorn [2 ]
Botanga, Christopher J. [3 ,4 ]
Thorgrimsen, Stephan [1 ]
Palma, Kristoffer [1 ]
Suarez-Rodriguez, Maria Cristina [1 ]
Sandbech-Clausen, Signe [1 ]
Lichota, Jacek [5 ]
Brodersen, Peter [6 ]
Grasser, Klaus D. [5 ]
Mattsson, Ole [1 ]
Glazebrook, Jane [3 ,4 ]
Mundy, John [1 ]
Petersen, Morten [1 ]
机构
[1] Univ Copenhagen, Dept Biol, DK-2200 Copenhagen, Denmark
[2] Tech Univ Denmark, Dept Syst Biol, Ctr Biol Sequence Anal, Kongens Lyngby, Denmark
[3] Univ Minnesota, Dept Plant Biol, St Paul, MN 55108 USA
[4] Univ Minnesota, Ctr Microbiol & Plant Genom, St Paul, MN 55108 USA
[5] Aalborg Univ, Dept Life Sci, Aalborg, Denmark
[6] CNRS, Inst Biol Mol Plantes, Unite Propre Rech 2357, F-67084 Strasbourg, France
关键词
Arabidopsis; defence response; MAP kinase; target genes; transcription factor;
D O I
10.1038/emboj.2008.147
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Plant and animal perception of microbes through pathogen surveillance proteins leads to MAP kinase signalling and the expression of defence genes. However, little is known about how plant MAP kinases regulate specific gene expression. We report that, in the absence of pathogens, Arabidopsis MAP kinase 4 (MPK4) exists in nuclear complexes with the WRKY33 transcription factor. This complex depends on the MPK4 substrate MKS1. Challenge with Pseudomonas syringae or flagellin leads to the activation of MPK4 and phosphorylation of MKS1. Subsequently, complexes with MKS1 and WRKY33 are released from MPK4, and WRKY33 targets the promoter of PHYTOALEXIN DEFICIENT3 (PAD3) encoding an enzyme required for the synthesis of antimicrobial camalexin. Hence, wrky33 mutants are impaired in the accumulation of PAD3 mRNA and camalexin production upon infection. That WRKY33 is an effector of MPK4 is further supported by the suppression of PAD3 expression in mpk4-wrky33 double mutant backgrounds. Our data establish direct links between MPK4 and innate immunity and provide an example of how a plant MAP kinase can regulate gene expression by releasing transcription factors in the nucleus upon activation.
引用
收藏
页码:2214 / 2221
页数:8
相关论文
共 44 条
[1]   The MAP kinase substrate MKS1 is a regulator of plant defense responses [J].
Andreasson, E ;
Jenkins, T ;
Brodersen, P ;
Thorgrimsen, S ;
Petersen, NHT ;
Zhu, SJ ;
Qiu, JL ;
Micheelsen, P ;
Rocher, A ;
Petersen, M ;
Newman, MA ;
Nielsen, HB ;
Hirt, H ;
Somssich, I ;
Mattsson, O ;
Mundy, J .
EMBO JOURNAL, 2005, 24 (14) :2579-2589
[2]   MAP kinase signalling cascade in Arabidopsis innate immunity [J].
Asai, T ;
Tena, G ;
Plotnikova, J ;
Willmann, MR ;
Chiu, WL ;
Gomez-Gomez, L ;
Boller, T ;
Ausubel, FM ;
Sheen, J .
NATURE, 2002, 415 (6875) :977-983
[3]   Salicylic acid-independent ENHANCED DISEASE SUSCEPTIBILITY1 signaling in Arabidopsis immunity and cell death is regulated by the monooxygenase FMO1 and the nudix hydrolase NUDT7 [J].
Bartsch, M ;
Gobbato, E ;
Bednarek, P ;
Debey, S ;
Schultze, JL ;
Bautor, J ;
Parker, JE .
PLANT CELL, 2006, 18 (04) :1038-1051
[4]   The MAP kinase kinase MKK2 affects disease resistance in Arabidopsis [J].
Brader, Gunter ;
Djamei, Armin ;
Teige, Markus ;
Palva, E. Tapio ;
Hirt, Heribert .
MOLECULAR PLANT-MICROBE INTERACTIONS, 2007, 20 (05) :589-596
[5]   Arabidopsis MAP kinase 4 regulates salicylic acid- and jasmonic acid/ethylene-dependent responses via EDS1 and PAD4 [J].
Brodersen, Peter ;
Petersen, Morten ;
Nielsen, Henrik Bjorn ;
Zhu, Shijiang ;
Newman, Mari-Anne ;
Shokat, Kevan M. ;
Rietz, Steffen ;
Parker, Jane ;
Mundy, John .
PLANT JOURNAL, 2006, 47 (04) :532-546
[6]   Phosphorylation sites of Arabidopsis MAP kinase substrate 1 (MKS 1) [J].
Caspersen, Mikael B. ;
Qiu, Jin-Long ;
Zhang, Xumin ;
Andreasson, Erik ;
Naested, Henrik ;
Mundy, John ;
Svensson, Birte .
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 2007, 1774 (09) :1156-1163
[7]   Characterization of the cyclophilin gene family of Arabidopsis thaliana and phylogenetic analysis of known cyclophilin proteins [J].
Chou, IT ;
Gasser, CS .
PLANT MOLECULAR BIOLOGY, 1997, 35 (06) :873-892
[8]   TLR-independent control of innate immunity in Caenorhabditis elegans by the TIR domain adaptor protein TIR-1, an ortholog of human SARM [J].
Couillault, C ;
Pujol, N ;
Reboul, J ;
Sabatier, L ;
Guichou, JF ;
Kohara, Y ;
Ewbank, JJ .
NATURE IMMUNOLOGY, 2004, 5 (05) :488-494
[9]   Plant NBS-LRR proteins in pathogen sensing and host defense [J].
DeYoung, Brody J. ;
Innes, Roger W. .
NATURE IMMUNOLOGY, 2006, 7 (12) :1243-1249
[10]   Transcriptional regulation of β-defensin gene expression in tracheal epithelial cells [J].
Diamond, G ;
Kaiser, V ;
Rhodes, J ;
Russell, JP ;
Bevins, CL .
INFECTION AND IMMUNITY, 2000, 68 (01) :113-119