The prolactin inhibition of follicle-stimulating hormone-induced aromatase activity in cultured rat granulosa cells is in part tyrosine kinase and protein kinase-C dependent

被引:13
作者
Alberto Villanueva, Luis [1 ]
Mendez, Isabel [1 ]
Ampuero, Sandra [2 ]
Larrea, Fernando [1 ]
机构
[1] Inst Nacl Nutr Salvador Zubiran, Dept Biol Reprod, Vasco de Quiroga 15, Mexico City 14000, DF, Mexico
[2] Pontificia Univ Catolica Chile, Fac Ciencias Biol, Unidad Reprod & Desarrollo, Santiago, Chile
关键词
ovary; prolactin; protein kinases; steroidogenesis;
D O I
10.1093/molehr/2.10.725
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The inhibitory actions of prolactin on gonadal steroidogenesis have been reported in different species and under a variety of experimental approaches. In this study, the mechanisms of the in-vitro effects of human prolactin (hPRL) on human follicle stimulating hormone (hFSH)-induced aromatase activity were determined using cultured granulosa cells from diethylstilboestrol (DES)-primed immature rats. Human PRL caused a dose-dependent decrease in hFSH-induced 17 beta-oestradiol production, even when cells were cultured in the presence of a cAMP analogue (8-Br-cAMP). These effects of hPRL appeared to be specific, since addition of an anti-rat PRL receptor monoclonal antibody (mAb) mimicked the hPRL inhibitory effect upon steroidogenesis in rat granulosa cells. In order to assess the importance of tyrosine kinase and protein kinase-C activation in the hPRL inhibitory effects upon oestrogen biosynthesis, cells were cultured in the presence of kinase inhibitors. The results showed that addition of genistein or staurosporine (a tyrosine kinase and protein kinase-C antagonist respectively) to cultured granulosa cells resulted in potent inhibition of hPRL actions upon hFSH-induced aromatization in a dose-dependent manner. These observations suggest that tyrosine kinase and protein kinase-C activation are involved in the biochemical events leading to hPRL inhibitory effects at the gonadal level.
引用
收藏
页码:725 / 731
页数:7
相关论文
共 40 条
[1]   PROLACTIN-INDUCED PROTEIN-KINASE-C ACTIVITY IN A MOUSE MAMMARY CELL-LINE (NOG-8) [J].
BANERJEE, R ;
VONDERHAAR, BK .
MOLECULAR AND CELLULAR ENDOCRINOLOGY, 1992, 90 (01) :61-67
[2]   PROLACTIN AND ANTIPROLACTIN RECEPTOR ANTIBODY INHIBIT STEROIDOGENESIS BY PURIFIED RAT LEYDIG-CELLS IN CULTURE [J].
BARKEY, RJ ;
WEISSMESSER, E ;
MANDEL, S ;
GAHNEM, F ;
AMIT, T .
MOLECULAR AND CELLULAR ENDOCRINOLOGY, 1987, 52 (1-2) :71-80
[3]   RAPID ACTIVATION OF PROTEIN KINASE-C IN ISOLATED RAT-LIVER NUCLEI BY PROLACTIN, A KNOWN HEPATIC MITOGEN [J].
BUCKLEY, AR ;
CROWE, PD ;
RUSSELL, DH .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (22) :8649-8653
[4]   INHIBITION BY GENISTEIN OF PROLACTIN-INDUCED NB2 LYMPHOMA CELL MITOGENESIS [J].
BUCKLEY, AR ;
BUCKLEY, DJ ;
GOUT, PW ;
LIANG, HQ ;
RAO, YP ;
BLAKE, MJ .
MOLECULAR AND CELLULAR ENDOCRINOLOGY, 1993, 98 (01) :17-25
[5]   PROLACTIN DIRECTLY INHIBITS BASAL AS WELL AS GONADOTROPIN-STIMULATED SECRETION OF PROGESTERONE AND 17-BETA-ESTRADIOL IN THE HUMAN-OVARY [J].
DEMURA, R ;
ONO, M ;
DEMURA, H ;
SHIZUME, K ;
OOUCHI, H .
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1982, 54 (06) :1246-1250
[6]   PROLACTIN STIMULATION OF PROTEIN-KINASE-C ACTIVITY IN THE RAT HYPOTHALAMUS [J].
DEVITO, WJ ;
STONE, S ;
AVAKIAN, C .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1991, 176 (02) :660-667
[7]   PROLACTIN INHIBITS ESTROGEN SYNTHESIS IN THE OVARY [J].
DORRINGTON, J ;
GORELANGTON, RE .
NATURE, 1981, 290 (5807) :600-602
[8]   ANTI-GONADAL ACTION OF PROLACTIN - FURTHER-STUDIES ON THE MECHANISM OF INHIBITION OF FOLLICLE-STIMULATING HORMONE-INDUCED AROMATASE-ACTIVITY IN RAT GRANULOSA-CELL CULTURES [J].
DORRINGTON, JH ;
GORELANGTON, RE .
ENDOCRINOLOGY, 1982, 110 (05) :1701-1707
[9]  
ELBERG G, 1990, J BIOL CHEM, V265, P14770
[10]   SITES OF INHIBITION OF STEROIDOGENESIS BY ACTIVATION OF PROTEIN-KINASE-C IN SWINE OVARIAN (GRANULOSA) CELLS [J].
FLORES, JA ;
GARMEY, JC ;
NESTLER, JE ;
VELDHUIS, JD .
ENDOCRINOLOGY, 1993, 132 (05) :1983-1990