If You Don't Look, You Won't See: Intravital Multiphoton Imaging of Primary and Metastatic Breast Cancer

被引：16

作者：

Bonapace, Laura ^{[1
,2
]}

Wyckoff, Jeffrey ^{[1
]}

Oertner, Thomas ^{[1
]}

Van Rheenen, Jacco ^{[3
,4
]}

Junt, Tobias ^{[2
]}

Bentires-Alj, Mohamed ^{[1
]}

机构：

[1] Friedrich Miescher Inst Biomed Res FMI, CH-4058 Basel, Switzerland

[2] Novartis Inst Biomed Res, Basel, Switzerland

[3] Hubrecht Inst KNAW, Utrecht, Netherlands

[4] Univ Med Ctr Utrecht, Utrecht, Netherlands

来源：

JOURNAL OF MAMMARY GLAND BIOLOGY AND NEOPLASIA | 2012年 / 17卷 / 02期

基金：

欧洲研究理事会;

关键词：

Multiphoton microscopy; Intravital imaging; Breast cancer; Metastasis; TUMOR-CELLS; MICROSCOPY; INVASION; INSIGHTS;

D O I：

10.1007/s10911-012-9250-8

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

A fundamental hallmark of cancer is progression to metastasis and the growth of breast cancer metastases in lung, bone, liver and/or brain causes fatal complications. Unfortunately, the cellular and biochemical mechanisms of the metastatic process remain ill-defined. Recent application of intravital multiphoton microscopy (MP-IVM) to image fluorescently labeled cells in mouse models of cancer has allowed dynamic observation of this multi-step process at the cellular and subcellular levels. In this article, we discuss the use of MP-IVM in studies of breast cancer metastasis, as well as surgical techniques for exposing tumors prior to imaging. We also describe a versatile multiphoton microscope for imaging tumor-stroma interactions.

引用

页码：125 / 129

页数：5

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