Automated system for capture and detection of nucleic acids

被引：3

作者：

Brown, A ^{[1
]}

Akinsanya, AA ^{[1
]}

Barker, SJ ^{[1
]}

Brophy, M ^{[1
]}

Dobb, AK ^{[1
]}

Doyle, SM ^{[1
]}

Hudson, IR ^{[1
]}

Minter, SJ ^{[1
]}

Wraith, MJ ^{[1
]}

Oultram, JD ^{[1
]}

机构：

[1] Tepnel Life Sci PLC, Manchester M20 2RY, Lancs, England

来源：

BIOTECHNIQUES | 1999年 / 27卷 / 01期

关键词：

D O I：

10.2144/99271pf01

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A fully automated nucleic acid analysis system is described, which offers positive sample identification, improved sensitivity and reduced user interaction compared to conventional techniques. The system relies on the sequence-specific capture of DNA onto solid-phase particles, confirming product identity without the problems of interpretation and lack of sequence information inherent in gel-based analyses. The system can be used for sequence confirmation, mutation analysis and semiquantitative detection of PCR products.

引用

页码：176 / 180

页数：5