Post-SELEX combinatorial optimization of aptamers

被引：82

作者：

Eaton, BE ^{[1
]}

Gold, L ^{[1
]}

Hicke, BJ ^{[1
]}

Janjic, N ^{[1
]}

Jucker, FM ^{[1
]}

Sebesta, DP ^{[1
]}

Tarasow, TM ^{[1
]}

Willis, MC ^{[1
]}

Zichi, DA ^{[1
]}

机构：

[1] NEXSTAR PHARMACEUT INC,BOULDER,CO 80301

来源：

BIOORGANIC & MEDICINAL CHEMISTRY | 1997年 / 5卷 / 06期

关键词：

D O I：

10.1016/S0968-0896(97)00044-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In vitro selection techniques provide a means of isolating nucleic acid ligands for binding to particular protein targets. Although most aptamers have quite high affinities for their target proteins, it has been shown that post-SELEX modification can result in further enhancement of binding affinity, as well as other desired properties. This has led to the current development of a more systematic approach to aptamer optimization using a combinatorial screening methodology. (C) 1997 Elsevier Science Ltd.

引用

页码：1087 / 1096

页数：10

共 46 条

[1]

Austin D J, 1994, Chem Biol, V1, P131, DOI 10.1016/1074-5521(94)90002-7

[2] ISOLATION OF NEW RIBOZYMES FROM A LARGE POOL OF RANDOM SEQUENCES [J].