Functional analysis of phenylalanine 365 in hopene synthase, a conserved amino acid in the families of squalene and oxidosqualene cyclases

被引：44

作者：

Hoshino, T ^{[1
]}

Sato, T

机构：

[1] Niigata Univ, Fac Agr, Dept Appl Biol Chem, Niigata 9502181, Japan

[2] Niigata Univ, Grad Sch Sci & Technol, Niigata 9502181, Japan

来源：

CHEMICAL COMMUNICATIONS | 1999年 / 19期

关键词：

D O I：

10.1039/a905559b

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Two bicyclic products were accumulated by the mutant F365A, showing the amino acid residue is located close to the transient C-8 carbocation intermediate in the active site cavity; the mutants of F365Y and F365W significantly accelerated the cyclization reaction at low temperatures.

引用

页码：2005 / 2006

页数：2

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