An antifungal exo-α-1,3-glucanase (AGN13.1) from the biocontrol fungus Trichoderma harzianum

被引：111

作者：

Ait-Lahsen, H

Soler, A

Rey, M

de la Cruz, J

Monte, E

Llobell, A

机构：

[1] Univ Seville, CSIC, Inst Bioquim Vegetal & Fotosintesis, Ctr Invest Cient Isla de la Cartuja, E-41092 Seville, Spain

[2] Univ Salamanca, Ctr Hispano Luso Invest Agr, E-37008 Salamanca, Spain

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2001年 / 67卷 / 12期

关键词：

D O I：

10.1128/AEM.67.12.5833-5839.2001

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Trichoderma harzianum secretes alpha -1,3-glucanases when it is grown on polysaccharides, fungal cell walls, or autoclaved mycelium as a carbon source (simulated antagonistic conditions). We have purified and characterized one of these enzymes, named AGN13.1. The enzyme was monomeric and slightly basic. AGN13.1 was an exo-type alpha -1,3-glucanase and showed lytic and antifungal activity against fungal plant pathogens. Northern and Western analyses indicated that AGN13.1 is induced by conditions that simulated antagonism. We propose that AGN13.1 contributes to the antagonistic response of T. harzianum.

引用

页码：5833 / 5839

页数：7

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