WbjA adds glucose to complete the O-antigen trisaccharide repeating unit of the lipopolysaccharide of Pseudomonas aeruginosa serogroup O11

被引:20
作者
Dean, CR
Datta, A
Carlson, RW
Goldberg, JB
机构
[1] Univ Virginia, Hlth Sci Ctr, Dept Microbiol, Charlottesville, VA 22908 USA
[2] Univ Georgia, Complex Carbohydrate Res Ctr, Athens, GA 30602 USA
关键词
D O I
10.1128/JB.184.1.323-326.2002
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Lipopolysaccharide from a wbjA mutant, deficient in a putative glycosyltransferase from Pseudomonas aeruginosa serogroup O11, was compared to that from an O-antigen polymerase mutant. Results suggest that WbjA adds the terminal glucose to complete the serogroup O11 O-antigen unit and identifies the biological repeating unit as [-2)-beta -D-glucose-(1-3)-alpha -L-N-acetylfucosamine-(1-3)-beta -D-N-acetylfucosamine-(1].
引用
收藏
页码:323 / 326
页数:4
相关论文
共 25 条
[1]   Functional analysis of genes responsible for the synthesis of the B-band O antigen of Pseudomonas aeruginosa serotype O6 lipopolysaccharide [J].
Bélanger, M ;
Burrows, LL ;
Lam, JS .
MICROBIOLOGY-UK, 1999, 145 :3505-3521
[2]   Molecular characterization of the Pseudomonas aeruginosa serotype O5 (PAO1) B-band lipopolysaccharide gene cluster [J].
Burrows, LL ;
Charter, DF ;
Lam, JS .
MOLECULAR MICROBIOLOGY, 1996, 22 (03) :481-495
[3]   Characterization of the serogroup O11O-antigen locus of Pseudomonas aeruginosa PA103 [J].
Dean, CR ;
Franklund, CV ;
Retief, JD ;
Coyne, MJ ;
Hatano, K ;
Evans, DJ ;
Pier, GB ;
Goldberg, JB .
JOURNAL OF BACTERIOLOGY, 1999, 181 (14) :4275-4284
[4]  
DMITRIEV BA, 1980, EUR J BIOCHEM, V106, P643, DOI 10.1111/j.1432-1033.1980.tb04612.x
[5]   The activity of a putative polyisoprenol-linked sugar translocase (Wzx) involved in Escherichia coli O antigen assembly is independent of the chemical structure of the O repeat [J].
Feldman, MF ;
Marolda, CL ;
Monteiro, MA ;
Perry, MB ;
Parodi, AJ ;
Valvano, MA .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (49) :35129-35138
[6]   CLONING AND SURFACE EXPRESSION OF PSEUDOMONAS-AERUGINOSA O-ANTIGEN IN ESCHERICHIA-COLI [J].
GOLDBERG, JB ;
HATANO, K ;
MELULENI, GS ;
PIER, GB .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (22) :10716-10720
[7]   FUNCTION OF THE RFB GENE-CLUSTER AND THE RFE GENE IN THE SYNTHESIS OF O-ANTIGEN BY SHIGELLA-DYSENTERIAE-1 [J].
KLENA, JD ;
SCHNAITMAN, CA .
MOLECULAR MICROBIOLOGY, 1993, 9 (02) :393-402
[8]  
KNIREL YA, 1994, BIOCHEMISTRY-MOSCOW+, V59, P1325
[9]   POLYSACCHARIDE ANTIGENS OF PSEUDOMONAS-AERUGINOSA [J].
KNIREL, YA .
CRITICAL REVIEWS IN MICROBIOLOGY, 1990, 17 (04) :273-304
[10]   Expression and study of recombinant ExoM, a β1-4 glucosyltransferase involved in succinoglycan biosynthesis in Sinorhizobium meliloti [J].
Lellouch, AC ;
Geremia, RA .
JOURNAL OF BACTERIOLOGY, 1999, 181 (04) :1141-1148