The Azospirillum brasilense rpoN gene is involved in nitrogen fixation, nitrate assimilation, ammonium uptake, and flagellar biosynthesis

被引:29
作者
Milcamps, A
VanDommelen, A
Stigter, J
Vanderleyden, J
deBruijn, FJ
机构
[1] MICHIGAN STATE UNIV,US DOE,PLANT RES LAB,E LANSING,MI 48824
[2] MICHIGAN STATE UNIV,NATL SCI FDN,CTR MICROBIAL ECOL,E LANSING,MI 48824
[3] KATHOLIEKE UNIV LEUVEN,FA JANSSENS LAB GENET,B-3001 HEVERLEE,BELGIUM
[4] MICHIGAN STATE UNIV,DEPT MICROBIOL,E LANSING,MI 48824
关键词
Azospirillum brasilense; sigma factor; nitrogen fixation; ammonium assimilation; motility;
D O I
10.1139/m96-064
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The rpoN (ntrA) gene (encoding sigma 54) of Azospirillum brasilense Sp7 was isolated by using conserved rpoN primers and the polymerase chain reaction. and its nucleotide sequence was determined. The deduced amino acid sequence of the RpoN protein was found to share a high degree of homology with other members of the sigma 54 family. Two additional open reading frames were found in the Azospirillum brasilense rpoN region, with significant similarity to equivalent regions surrounding the rpoN locus in other bacteria. An rpoN mutant of Azospirillum brasilense Sp7 was constructed by gene replacement and found to be defective in nitrogen fixation, nitrate assimilation, and ammonium uptake. Lack of ammonium uptake was also found in previously isolated Azospirillum brasilense ntrB and ntrC mutants, further supporting the role of the ntr system in this process. In addition, the rpoN mutant was found to be nonmotile, suggesting a role of RpoN in Azospirillum brasilense flagellar biosynthesis.
引用
收藏
页码:467 / 478
页数:12
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