Hsp70 family member, mot-2/mthsp70/GRP75, binds to the cytoplasmic sequestration domain of the p53 protein

被引:146
作者
Wadhwa, R
Yaguchi, T
Hasan, K
Mitsui, Y
Reddel, RR
Kaul, SC
机构
[1] Natl Inst Adv Ind Sci & Technol, Inst Mol & Cell Biol, Tsukuba, Ibaraki 3058566, Japan
[2] Chugai Res Inst Med Sci, Niihari, Ibaraki 30041, Japan
[3] Childrens Med Res Inst, Westmead, NSW 2145, Australia
关键词
mot-2/mthsp70; p53; binding domain;
D O I
10.1006/excr.2002.5468
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Hsp70 family member mot-2/mthsp70/GRP75/PBP74 was shown to bind to the tumor suppressor protein p53. In this study, by an vivo coimmunoprecipitation of mot-2 with p53 and its deletion mutants, the mot-2 binding site of p53 was mapped to its C-terminal amino acid residues 312-352, a region of p53 that includes its cytoplasmic sequestration domain. These data demonstrate that cytoplasmic sequestration and inactivation of p53 by mot-2 occurs by its binding to the cytoplasmic sequestration domain. Therefore, perturbation of mot-p53 interactions can be employed to abrogate cytoplasmic retention of wild-type p53 in tumors. (C) 2002 Elsevier Science (USA).
引用
收藏
页码:246 / 253
页数:8
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