Development of an Indirect Enzyme Immunoassay using Monoclonal Antibodies for the Measurement of 17-Hydroxyprogesterone in Human Serum

被引：4

作者：

Chong, Heilly ^{[1
]}

Cheah, Swee Hung ^{[1
]}

Ragavan, M. ^{[1
]}

Johgalingam, V. T. ^{[1
]}

机构：

[1] Univ Malaya, Fac Med, Dept Physiol, Monoclonal Lab, Kuala Lumpur 50603, Malaysia

来源：

JOURNAL OF IMMUNOASSAY & IMMUNOCHEMISTRY | 2009年 / 30卷 / 02期

基金：

美国国家科学基金会;

关键词：

17-Hydroxyprogesterone; Indirect enzyme immunoassay; Monoclonal antibody; CONGENITAL ADRENAL-HYPERPLASIA; SOLID-PHASE RADIOIMMUNOASSAY; ANDROGEN-BINDING PROTEIN; 21-HYDROXYLASE DEFICIENCY; 17-ALPHA-HYDROXYPROGESTERONE; EXPERIENCE; DIAGNOSIS; PLASMA;

D O I：

10.1080/15321810902782863

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

An indirect enzyme immunoassay for the measurement of total 17-hydroxyprogesterone (17OHP) in serum using monoclonal antibodies generated in our laboratory was developed. Here, (a) instead of extraction with solvents, serum was heated to free protein-bound 17OHP and assay was performed at pH 9.6, (b) to ensure uniform assay conditions for both standards and samples, buffer for standards contained charcoal-stripped pre-heated pooled cord serum. Assays were done in 96-well EIA microplates pre-coated with 17-hydroxyprogesterone-3-(o-carboxymethyl)oxime: bovine serum albumin. Secondary antibody was horseradish peroxidase-linked sheep anti-mouse IgG polyclonal antibody. The method was accurate and suitable for screening for congenital adrenal hyperplasia.

引用

页码：166 / 179

页数：14

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