Cryptosporidium parvum mixed genotypes detected by PCR-restriction fragment length polymorphism analysis

被引：29

作者：

Reed, C ^{[1
]}

Sturbaum, GD ^{[1
]}

Hoover, PJ ^{[1
]}

Sterling, CR ^{[1
]}

机构：

[1] Univ Arizona, Dept Vet Sci & Microbiol, Tucson, AZ 85721 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2002年 / 68卷 / 01期

关键词：

D O I：

10.1128/AEM.68.1.427-429.2002

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Combinations of 10 Cryptosporidium parvum oocysts, with various ratios of genotype I to genotype 11, were isolated and subjected to PCR-restriction fragment length polymorphism analysis. Amplification of both genotypes in these samples ranged from 31 to 74% and yielded no information about the genotype proportions. In addition, since both genotypes were not always detected, amplification of a single genotype is not conclusive evidence that the sample contains only a single genotype.

引用

页码：427 / 429

页数：3

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