The chloroplast psbA promoter is more efficient in Escherichia coli than the T7 promoter for hyperexpression of a foreign protein

被引：26

作者：

Brixey, PJ ^{[1
]}

Guda, C ^{[1
]}

Daniell, H ^{[1
]}

机构：

[1] AUBURN UNIV,DEPT BOT & MICROBIOL,MOL GENET PROGRAM,AUBURN,AL 36849

来源：

BIOTECHNOLOGY LETTERS | 1997年 / 19卷 / 04期

基金：

美国国家卫生研究院;

关键词：

HIGH-LEVEL EXPRESSION; PLANTS; GENES;

D O I：

10.1023/A:1018371405675

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The T7 promoter is commonly used in E. coli expression studies but has several disadvantages due to induction with isopropylthio-beta-D-galactoside (IPTG) including the prohibitive cost of IPTG, reduction in cell growth, and the poisonous nature of IPTG prohibiting its use in pharmaceutical products. The chloroplast psbA promoter produces an 18.5 fold increase in expression in E. coli compared to the T7 promoter. Also, the psbA promoter does not have the disadvantages of induction with IPTG.

引用

页码：395 / 399

页数：5

共 16 条

[1] CHARACTERIZATION OF P-SATIVUM CHLOROPLAST PSBA TRANSCRIPTS PRODUCED INVIVO, INVITRO AND IN ESCHERICHIA-COLI [J].

BOYER, SK ;

MULLET, JE .

PLANT MOLECULAR BIOLOGY, 1986, 6 (04) :229-243

[2] NUCLEAR TRANSCRIPTIONAL ACTIVITY OF THE TOBACCO PLASTID PSBA PROMOTER [J].

CORNELISSEN, M ;

VANDEWIELE, M .

NUCLEIC ACIDS RESEARCH, 1989, 17 (01) :19-29

[3]

DANIELL H, 1993, METHOD ENZYMOL, V217, P536

[4] UPTAKE AND EXPRESSION OF BACTERIAL AND CYANOBACTERIAL GENES BY ISOLATED CUCUMBER ETIOPLASTS [J].

DANIELL, H ;

MCFADDEN, BA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (18) :6349-6353

[5]

DANIELL H, 1994, NATO ADV SCI INST SE, V86, P589

[6]

DANIELL H, 1996, METHOD MOL BIOL, V63, P359

[7] HYPER EXPRESSION OF AN ENVIRONMENTALLY FRIENDLY SYNTHETIC-POLYMER GENE [J].

GUDA, C ;

ZHANG, X ;

MCPHERSON, DT ;

XU, J ;

CHERRY, JH ;

URRY, DW ;

DANIELL, H .

BIOTECHNOLOGY LETTERS, 1995, 17 (07) :745-750

[8] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[9] COPPER STAINING - A 5-MINUTE PROTEIN STAIN FOR SODIUM DODECYL-SULFATE POLYACRYLAMIDE GELS [J].

LEE, C ;

LEVIN, A ;

BRANTON, D .

ANALYTICAL BIOCHEMISTRY, 1987, 166 (02) :308-312

[10]

LEWIN B, 1990, GENES, V4, P416

← 1 2 →