Effect of Hypoxia on Neural Induction in Colonies of Human Parthenogenetic Stem Cells

被引：4

作者：

Abramihina, T. V. ^{[1
]}

Isaev, D. A. ^{[1
]}

Semechkin, R. A. ^{[1
,2
]}

机构：

[1] Int Stem Cell Corp, Carlsbad, CA USA

[2] Russian Acad Med Sci, PK Anokhin Inst Normal Physiol, Moscow, Russia

来源：

BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE | 2012年 / 154卷 / 01期

关键词：

neural induction; parthenogenetic stem cells; hypoxia; OXYGEN-TENSIONS; DIFFERENTIATION; PLURIPOTENCY; BLASTOCYSTS;

D O I：

10.1007/s10517-012-1892-4

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

100103 [病原生物学]; 100218 [急诊医学];

摘要：

We studied neural induction and generation of neuroectoderm in the colonies of human parthenogenetic SC cultured in the presence of 5 and 19 +/- 2% oxygen. We found that neuroectoderm was more actively generated at high oxygen content. At the same time, the transcription of stem cell pluripotency genes was not completely suppressed during neural induction at low oxygen content, while the expression of endoderm and mesodermal marker genes attested to the absence of specifi c differentiation. These fi ndings demonstrate more effi cient neuroectoderm generation induced in the colonies of pluripotent stem cells under conditions of normoxia.

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页码：130 / 132

页数：3

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