Processing of the Bacillus subtilis thrS leader mRNA is RNase E-dependent in Escherichia coli

被引：56

作者：

Condon, C ^{[1
]}

Putzer, H ^{[1
]}

Luo, D ^{[1
]}

GrunbergManago, M ^{[1
]}

机构：

[1] INST BIOL PHYSICOCHIM,UPR 9073,F-75005 PARIS,FRANCE

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1997年 / 268卷 / 02期

关键词：

B-subtilis; threonyl-tRNA synthetase; mRNA processing; RNase E; endoribonuclease;

D O I：

10.1006/jmbi.1997.0971

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have recently reported that processing occurs in the untranslated leader region of several members of a family of Gram-positive genes regulated by tRNA-mediated antitermination. We showed that cleavage at this site plays an important role in the induction of Bacillus subtilis thrS gene expression, following threonine starvation, by stabilising the downstream mRNA. Here we show that, when transferred on a plasmid, processing of the B. subtilis thrS leader can occur at the same site in Escherichia coli. Cleavage at this site is dependent on the E. coli endoribonuclease E, both in vivo and in vitro, suggesting that a functional homologue of RNase E is responsible for thrS processing in B. subtilis. (C) 1997 Academic Press Limited.

引用

页码：235 / 242

页数：8

共 52 条

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