Transplantation of Adipose-Derived Stem Cells is Associated with Neural Differentiation and Functional Improvement in a Rat Model of Intracerebral Hemorrhage

被引：73

作者：

Chen, Juan ^{[1
,2
]}

Tang, Ying-Xin ^{[1
]}

Liu, Yong-Ming ^{[1
]}

Chen, Ji ^{[3
,4
]}

Hu, Xiao-Qing ^{[1
]}

Liu, Na ^{[1
]}

Wang, Shu-Xin ^{[1
]}

Zhang, Yu ^{[1
]}

Zeng, Wen-Gao ^{[1
]}

Ni, Hou-Jie ^{[1
]}

Zhao, Bin ^{[3
]}

Chen, Yan-Fang ^{[3
,4
]}

Tang, Zhou-Ping ^{[1
]}

机构：

[1] Huazhong Univ Sci & Technol, Dept Neurol, Tongji Hosp, Tongji Med Coll, Wuhan 430030, Hubei, Peoples R China

[2] Univ Hosp, Dept Neurol, Hubei Inst Nationalities, Enshi, Peoples R China

[3] Guangdong Med Coll, Affiliated Hosp, Dept Neurol, Clin Res Ctr, Zhanjiang, Peoples R China

[4] Wright State Univ, Dept Pharmacol & Toxicol, Boonshoft Sch Med, Dayton, OH 45435 USA

来源：

CNS NEUROSCIENCE & THERAPEUTICS | 2012年 / 18卷 / 10期

基金：

中国国家自然科学基金;

关键词：

Apoptosis; Hemorrhagic stroke; Progenitor cells; Rat; Transplantation; ENDOTHELIAL GROWTH-FACTOR; CEREBRAL-ARTERY OCCLUSION; STROMAL CELLS; NEUROLOGICAL-DISORDERS; IN-VITRO; NEUROGENIC DIFFERENTIATION; STROKE MODEL; BRAIN EDEMA; BONE-MARROW; TISSUE;

D O I：

10.1111/j.1755-5949.2012.00382.x

中图分类号：

Q189 [神经科学];

学科分类号：

071006 [神经生物学];

摘要：

Aims To examine whether transplantation of adipose-derived stem cells (ADSCs) induces neural differentiation and improves neural function in a rat intracerebral hemorrhage (ICH) model. Methods Adipose-derived stem cells cells were isolated from inguinal fat pad of rat. ICH was induced by injection of collagenase type IV into the right basal ganglia of rat. Forty-eight hours after ICH, ADSCs cells (10 mu L of 24 x 107 cells/mL) were injected into the right lateral cerebral ventricle. The differentiation of ADSCs was detected in vitro and in vivo. The neural function was evaluated with Zea Longa 5-grade scale at day 1, 3, 7, 14, or 28. Results Our data demonstrated that ADSCs differentiated into cells that shared the similarities of neurons or astrocytes in vitro. Transplantation of ADSCs decreased cell apoptosis and the transplanted ADSCs were able to differentiate into neuron-like and astrocyte-like cells around the hematoma, accompanied with upregulation of vascular endothelial growth factor expression and improvement of neural function. Conclusions Our data suggest that transplantation of ADSCs could be a therapeutic approach for ICH stroke.

引用

页码：847 / 854

页数：8

共 36 条

[1]

Sequential Hepatogenic Transdifferentiation of Adipose Tissue-Derived Stem Cells: Relevance of Different Extracellular Signaling Molecules, Transcription Factors Involved, and Expression of New Key Marker Genes [J].