APH-1, a POU homeobox gene expressed in the salt gland of the crustacean Artemia franciscana

被引:19
作者
Chavez, M
Landry, C
Loret, S
Muller, M
Figueroa, J
Peers, B
Rentier-Delrue, F
Rousseau, GG
Krauskopf, M
Martial, JA
机构
[1] Univ Liege, Lab Biol Mol & Genie Genet, B-4000 Sart Tilman Par Liege, Belgium
[2] Christian de Duve Inst Cellular Pathol, Hormone & Metab Res Unit, B-1200 Brussels, Belgium
[3] Louvain Univ, Sch Med, B-1200 Brussels, Belgium
[4] Univ Austral Chile, Fac Ciencias, Inst Bioquim, Valdivia, Chile
关键词
crustacean; Artemia; osmoregulation; homeodomain; POU protein; Cf1-a; POU-M1;
D O I
10.1016/S0925-4773(99)00152-5
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
We characterized the first POU-homeoprotein in a crustacean (designated APH-1 for Artemia POU-Homeoprotein, EMBL Y15070). The amino acid sequence of the APH-1 POU-domain is identical, except for two residues, to that of the two class III POU proteins Cf1-a (Drosophila) and POU-M1 (Bombyx mori). Southern blot analysis suggests that crustaceans have only one class III POU gene. RT-PCR and whole-mount in situ hybridization show that APH-1 mRNA is present in larvae specifically in the salt gland, an organ which is involved in osmoregulation, and disappears in the adult. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.
引用
收藏
页码:207 / 212
页数:6
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