Bacterial surface display of an anti-pollutant antibody fragment

被引：23

作者：

Dhillon, JK ^{[1
]}

Drew, PD ^{[1
]}

Porter, AJR ^{[1
]}

机构：

[1] Univ Aberdeen, Inst Med Sci, Dept Mol & Cell Biol, Aberdeen AB25 2ZD, Scotland

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1999年 / 28卷 / 05期

关键词：

D O I：

10.1046/j.1365-2672.1999.00552.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A peptidoglycan-associated lipoprotein (PAL) fused to an antibody fragment (scFv) specific to the herbicide and environmental pollutant atrazine, has been successfully targeted to the cell surface of Escherichia toll. Anti-atrazine binding could be observed via an atrazine-alkaline phosphatase conjugate. Cells containing the PAL fusion grew with little cellular toxicity when compared with the control. In contrast, expression of anti-atrazine antibody fragments alone caused the cells to lyse after 4 h. The surface display of anti-pollutant antibodies may have a future role in the bioremediation of contaminated water or the development of pollutant-specific, whole-cell biosensors.

引用

页码：350 / 354

页数：5

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