Functional, endogenously expressed 5-hydroxytryptamine 5-ht(7) receptors in human vascular smooth muscle cells

被引：39

作者：

Schoeffter, P ^{[1
]}

Ullmer, C ^{[1
]}

Bobirnac, I ^{[1
]}

Gabbiani, G ^{[1
]}

Lubbert, H ^{[1
]}

机构：

[1] FAC MED,DEPT PATHOL,CH-1211 GENEVA 4,SWITZERLAND

来源：

BRITISH JOURNAL OF PHARMACOLOGY | 1996年 / 117卷 / 06期

关键词：

5-HT receptors; 5-ht(7) receptors; vascular smooth muscle cells; cyclic AMP;

D O I：

10.1111/j.1476-5381.1996.tb16687.x

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Human uterine artery smooth muscle cells in culture were shown to express constitutively both 5-ht(7)-receptor mRNA and 5-ht(7)-like receptors functionally linked to cyclic AMP formation. 5-Carboxamidotryptamine (5-CT) and 5-HT enhanced forskolin-stimulated cyclic AMP accumulation in these cells, with pEC(50) values of 7.12 and 6.25, sumatriptan being very weakly active. Both methiothepin (0.1 mu M) and clozapine (1 mu M), but not the 5-HT4-receptor antagonist, SDZ 205-557 (10 mu M) antagonized the effects of 5-CT. In reverse transcriptase-polymerase chain reaction analysis, the mRNA for 5-ht(7), but not for 5-HT4 or 5-ht(6) receptors was found to be strongly expressed in the same cells. These findings represent a further step toward the recognition of 5-ht(7) receptors as real, functional receptors.

引用

页码：993 / 994

页数：2

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