Core-Shell Gold Nanoparticles as Non-Conventional Matrix for the MALDI-ToF-MS Detection of Amino Acids: A Preliminary Study

被引：6

作者：

Cioffi, N. ^{[1
,2
]}

De Palo, F. ^{[1
]}

Calvano, C. D. ^{[1
]}

van der Werf, I. D. ^{[1
]}

Palmisano, F. ^{[1
,2
]}

Zambonin, P. G. ^{[1
,2
]}

机构：

[1] Univ Bari, Dipartimento Chim, I-70126 Bari, Italy

[2] Univ Bari, Ctr Interdipartimentale SMART, I-70126 Bari, Italy

来源：

SENSOR LETTERS | 2008年 / 6卷 / 04期

关键词：

Gold Nanoparticle; MALDI; XPS; Amino Acid Detection; Proteomics;

D O I：

10.1166/sl.2008.454

中图分类号：

O65 [分析化学];

学科分类号：

070302 [分析化学]; 081704 [应用化学];

摘要：

Electrochemically synthesized nanoparticles (NPs) consisting of a gold core and a quaternary ammonium ("quat") shell were investigated as non-conventional matrix for the detection of low molecular weight analytes by Matrix Assisted Laser Desorption Ionization-Time of Flight-Mass Spectrometry (MALDI-ToF-MS). The performance level of pristine NPs in promoting the analyte desorption/ionization processes was affected by the surfactant excess, due to shadowing of gold. Additional electrochemical protocols were developed for the controlled deposition of NPs on MALDI plates, aiming to improve the surface availability of nanostructured gold. Before use in mass spectrometry, the active area was treated by a potentiostatic procedure, that was proven to remove the excess of quat from the NPs surface. MALDI-MS and X-ray Photoelectron Spectroscopy showed that electrodeposited NPs can proficiently interact with small analytes, like cysteine and 4-fluoro-phenylalanine.

引用

页码：654 / 661

页数：8

共 75 条

[1]

Fullerene non-linear excited state absorption induced by gold nanoparticles light harvesting [J].