A simple method for the synthesis of ceramides and radiolabeled analogues

被引：16

作者：

Anand, JK ^{[1
]}

Sadozai, KK ^{[1
]}

Hakomori, S ^{[1
]}

机构：

[1] BIOMEMBRANE INST,SEATTLE,WA 98119

来源：

LIPIDS | 1996年 / 31卷 / 09期

关键词：

D O I：

10.1007/BF02522695

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple method has been developed for the coupling of amines to carboxylic acids. N-Fatty-acyl-sphingosine, cerebroside, and GM(3), as well as their respective [C-14] analogues, were synthesized using diethylphosphoryl cyanide as a potent coupling agent in the presence of triethylamine. The reaction procedure is rapid, racemization-free, and utilizes acids without derivatization. The desired ceramide products were obtained in 85-90% yield within one hour. The facile method presented here can also be used to synthesize [H-3]-N-acyl-labeled, as well as [C-14] [H-3] double-labeled, ceramides.

引用

页码：995 / 998

页数：4

共 24 条

[1] N-HYDROXYSUCCINIMIDE ESTERS IN PEPTIDE SYNTHESIS [J].