A DNA microarray-based genetic screen for nonhomologous end-joining mutants in Saccharomyces cerevisiae

被引：115

作者：

Ooi, SL

Shoemaker, DD

Boeke, JD

机构：

[1] Johns Hopkins Univ, Sch Med, Dept Mol Biol & Genet, Baltimore, MD 21205 USA

[2] Rosetta Inpharmat Inc, Kirkland, WA 98034 USA

来源：

SCIENCE | 2001年 / 294卷 / 5551期

关键词：

D O I：

10.1126/science.1065672

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We describe a microarray-based screen performed by imposing different genetic selections on thousands of yeast mutants in parallel, representing most genes in the yeast genome. The presence or absence of mutants was detected by oligonucleotide arrays that hybridize to 20-nucleotide "barcodes." We used this method to screen for components of the nonhomologous end-joining (NHEJ) pathway. Known components of the pathway were identified, as well as a gene not previously known to be involved in NHEJ, NEJ1. Nej1 protein interacts with the amino terminus of LIF1/XRCC4, a recently recognized "guardian of the genome" against cancer.

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页码：2552 / 2556

页数：5

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