Identification of multiple plasmids released from recombinant genomes of Hansenula polymorpha by transformation of Escherichia coli

被引：10

作者：

Graupner, S ^{[1
]}

Wackernagel, W ^{[1
]}

机构：

[1] UNIV OLDENBURG,FACHBEREICH BIOL,D-26111 OLDENBURG,GERMANY

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1996年 / 62卷 / 05期

关键词：

D O I：

10.1128/AEM.62.5.1839-1841.1996

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Total DNAs isolated from two Hansenula polymorpha (Pichia angusta) strains having chromosomal single or tandem multiple integrations of a pUC18-derived expression plasmid produced Escherichia coli transformants which contained plasmids of different size and/or organization than that of the expression plasmid. Evidence that plasmid-like structures are formed in H. polymorpha and that their formation is stimulated by DNA damage is presented in this study.

引用

页码：1839 / 1841

页数：3

共 18 条

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