Green fluorescent protein as a reporter of gene expression in transgenic mice

被引：39

作者：

Chiocchetti, A ^{[1
]}

Tolosano, E ^{[1
]}

Hirsch, E ^{[1
]}

Silengo, L ^{[1
]}

Altruda, F ^{[1
]}

机构：

[1] UNIV TURIN,DEPT GENET BIOL & MED CHEM,I-10126 TURIN,ITALY

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1997年 / 1352卷 / 02期

关键词：

GFP; reporter gene; transgenic mouse; gene expression;

D O I：

10.1016/S0167-4781(97)00010-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We used the green fluorescent protein (GFP) from the jellyfish Aequorea victoria as a reporter of gene expression in transgenic mice. The GFP coding sequence was placed under the control of the human hemopexin and the mouse beta 1 integrin promoter that were previously studied in transgenic mice using the lacZ reporter gene. We showed that GFP has a higher degree of sensitivity compared to the lacZ reporter gene allowing to identify cells with low and otherwise undetectable beta-galactosidase activity. Thus we showed the potentiality of GFP in replacing lacZ as a reporter gene to investigate promoter mapping and gene regulation in transgenic mice. (C) 1997 Elsevier Science B.V.

引用

页码：193 / 202

页数：10

共 28 条

[1] Simultaneous fluorescence-activated cell sorter analysis of two distinct transcriptional elements within a single cell using engineered green fluorescent proteins [J].