Synthesis and characterization of 4-iodophenylboronic acid: A new enhancer for the horseradish peroxidase-catalyzed chemiluminescent oxidation of luminol

被引:37
作者
Kricka, JL [1 ]
Cooper, M [1 ]
Ji, XY [1 ]
机构
[1] TOCRIS COOKSON LTD,SOUTHAMPTON SO1 9JW,HANTS,ENGLAND
关键词
D O I
10.1006/abio.1996.0338
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
4-Iodophenylboronic acid has been synthesized and shown to be a potent enhancer of the chemiluminescent horseradish peroxidase (Type VI-A)-catalyzed oxidation of luminol and isoluminol. The enhancer was effective (>100-fold enhancement) in the concentration range 10-1500 mu M. Light emission in the presence of the enhancer peaked at 5-10 min after initiation of the reaction and then decayed very slowly. 4-Iodophenylboronic acid also enhanced reactions catalyzed by horseradish peroxidase Type IX. The detection limit for Type VI-A horseradish peroxidase was 509 amol, and optimum signal enhancement was obtained at 769 mu M compared to 154 mu M for 4-iodophenol under the same conditions. (C) 1996 Academic Press, Inc.
引用
收藏
页码:119 / 125
页数:7
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