Quantifying the fraction of glycine and alanine in β-sheet and helical conformations in spider dragline silk using solid-state NMR

被引：65

作者：

Holland, Gregory P. ^{[1
]}

Jenkins, Janelle E. ^{[1
]}

Creager, Melinda S. ^{[2
]}

Lewis, Randolph V. ^{[2
]}

Yarger, Jeffery L. ^{[1
]}

机构：

[1] Arizona State Univ, Dept Chem & Biochem, Magnet Resonance Res Ctr, Tempe, AZ 85287 USA

[2] Univ Wyoming, Dept Mol Biol, Laramie, WY 82071 USA

来源：

CHEMICAL COMMUNICATIONS | 2008年 / 43期

关键词：

D O I：

10.1039/b812928b

中图分类号：

O6 [化学];

学科分类号：

0703 [化学];

摘要：

Solid-state two-dimensional refocused INADEQUATE MAS NMR experiments resolve distinct helical and beta-sheet conformational environments for both alanine and glycine in Nephila clavipes dragline silk fibers; the fraction of alanine and glycine in beta-sheet structures is determined to be 82% +/- 4% and 28% +/- 5%, respectively.

引用

页码：5568 / 5570

页数：3

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