Integrin αvβ3 on human endothelial cells binds von Willebrand factor strings under fluid shear stress

被引:116
作者
Huang, Jing [1 ,2 ,3 ]
Roth, Robyn [4 ]
Heuser, John E. [4 ]
Sadler, J. Evan [1 ,2 ,3 ]
机构
[1] Washington Univ, Sch Med, Dept Med, St Louis, MO 63110 USA
[2] Washington Univ, Sch Med, Dept Biochem & Mol Biophys, St Louis, MO 63110 USA
[3] Washington Univ, Sch Med, Howard Hughes Med Inst, St Louis, MO 63110 USA
[4] Washington Univ, Sch Med, Dept Cell Biol, St Louis, MO 63110 USA
关键词
WEIBEL-PALADE BODIES; VONWILLEBRAND-FACTOR; FLOW CONDITIONS; P-SELECTIN; MONOCLONAL-ANTIBODY; SELF-ASSOCIATION; FACTOR MULTIMERS; ADHESION; FIBRONECTIN; ADAMTS-13;
D O I
10.1182/blood-2008-05-158584
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Acutely secreted von Willebrand factor (VWF) multimers adhere to endothelial cells, support platelet adhesion, and may induce microvascular thrombosis. Immunofluorescence microscopy of live human umbilical vein endothelial cells showed that VWF multimers rapidly formed strings several hundred micrometers long on the cell surface after stimulation with histamine. Unexpectedly, only a subset of VWF strings supported platelet binding, which depended on platelet glycoprotein Ib. Electron microscopy showed that VWF strings often consisted of bundles and networks of VWF multimers, and each string was tethered to the cell surface by a limited number of sites. Several approaches implicated P-selectin and integrin alpha(v)beta(3) in anchoring VWF strings. An RGDS peptide or a function-blocking antibody to integrin alpha(v)beta(3) reduced the number of VWF strings formed. In addition, integrin alpha(v) decorated the VWF strings by immunofluorescence microscopy. Furthermore, lentiviral transduction of shRNA against the alpha(v) subunit reduced the expression of cell-surface integrin alpha(v)beta(3) and impaired the ability of endothelial cells to retain VWF strings. Soluble P-selectin reduced the number of platelet-decorated VWF strings in the absence of Ca2+ and Mg2+ but had no effect in the presence of these cations. These results indicate that VWF strings bind specifically to integrin alpha(v)beta(3) on human endothelial cells. (Blood. 2009; 113: 1589-1597)
引用
收藏
页码:1589 / 1597
页数:9
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