Determination of glucose based on the effect of photons as a substitute for glucose oxidase

被引：17

作者：

Chen, M ^{[1
]}

Cai, WP ^{[1
]}

Zhu, QZ ^{[1
]}

Wang, XS ^{[1
]}

Xu, JG ^{[1
]}

机构：

[1] Xiamen Univ, Dept Chem, Res Lab SEDC Analyt Sci Mat & Life Chem, Xiamen 361005, Peoples R China

来源：

ANALYTICA CHIMICA ACTA | 1999年 / 388卷 / 1-2期

关键词：

photon; mimetic enzyme; fluorimetry; glucose determination;

D O I：

10.1016/S0003-2670(99)00130-0

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Glucose can produce H2O2 in solution when irradiated with UV light even in the absence of glucose oxidase. Based on this effect, photons were utilized as a substrate for glucose oxidase, and a new fluorescent method was developed for indirect determination of glucose by a coupled fluorescence reaction of p-hydroxyphenyl acetic acid and H2O2 with hemin as a catalyst. The calibration graph is linear over the range 0-6.0 x 10(-7) mol/l glucose, the detection limit is 1.7 x 10(-8) mol/l glucose. The method has been applied to the determination of glucose in human sera with satisfactory results. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：11 / 17

页数：7

共 19 条

[1] FLOW SPECTROPHOTOMETRIC METHOD FOR DETERMINATION OF HYDROGEN-PEROXIDE USING A CATION-EXCHANGER FOR PRECONCENTRATION
ALMUAIBED, AM
TOWNSHEND, A
[J]. ANALYTICA CHIMICA ACTA, 1994, 295 (1-2) : 159 - 163
[2] GENFA Z, 1992, ANAL CHEM, V64, P157
[3] Guilbault G. G., 1976, Handbook of Enzymatic Methods of Analysis
[4] HOMOVANILLIC ACID AS A FLUOROMETRIC SUBSTRATE FOR OXIDATIVE ENZYMES - ANALYTICAL APPLICATIONS OF PEROXIDASE GLUCOSE OXIDASE AND XANTHINE OXIDASE SYSTEMS
GUILBAULT, GG
BRIGNAC, P
ZIMMER, M
[J]. ANALYTICAL CHEMISTRY, 1968, 40 (01) : 190 - +
[5] NEW SUBSTRATES FOR FLUOROMETRIC DETERMINATION OF OXIDATIVE ENZYMES
GUILBAULT, GG
BRIGNAC, PJ
JUNEAU, M
[J]. ANALYTICAL CHEMISTRY, 1968, 40 (08) : 1256 - +
[6] FLUOROMETRIC FLOW-INJECTION DETERMINATION OF AQUEOUS PEROXIDES AT NANOMOLAR LEVEL USING MEMBRANE REACTORS
HWANG, H
DASGUPTA, PK
[J]. ANALYTICAL CHEMISTRY, 1986, 58 (07) : 1521 - 1524
[7] DEVELOPMENT OF A NOVEL CHEMILUMINESCENT PROBE, 4-(5',6'-DIMETHOXYBENZOTHIAZOLYL)PHTHALHYDRAZIDE
ISHIDA, J
TAKADA, M
HARA, S
SASAMOTO, K
KINA, K
YAMAGUCHI, M
[J]. ANALYTICA CHIMICA ACTA, 1995, 309 (1-3) : 211 - 219
[8] DEVELOPMENT OF A NOVEL LUMINOL-RELATED COMPOUND, 3-PROPYL-7,8-DIHYDROPYRIDAZINO[4,5-G]QUINOXALINE-2,6,9(1H)-TRIONE, AND ITS APPLICATION TO HYDROGEN-PEROXIDE AND SERUM GLUCOSE ASSAYS
ISHIDA, J
ARAKAWA, H
TAKADA, M
YAMAGUCHI, M
[J]. ANALYST, 1995, 120 (04) : 1083 - 1086
[9] THE USE OF GLUCOSE OXIDASE (NOTATIN) FOR THE DETERMINATION OF GLUCOSE IN BIOLOGICAL MATERIAL AND FOR THE STUDY OF GLUCOSE-PRODUCING SYSTEMS BY MANOMETRIC METHODS
KEILIN, D
HARTREE, EF
[J]. BIOCHEMICAL JOURNAL, 1948, 42 (02) : 230 - 238
[10] KELLY TA, 1991, ANAL CHEM, V63, P2110

← 1 2 →