Mgr3p and Mgr1p Are Adaptors for the Mitochondrial i-AAA Protease Complex

被引:44
作者
Dunn, Cory D. [1 ]
Tamura, Yasushi [1 ]
Sesaki, Hiromi [1 ]
Jensen, Robert E. [1 ]
机构
[1] Johns Hopkins Univ, Sch Med, Dept Cell Biol, Baltimore, MD 21205 USA
基金
美国国家卫生研究院;
关键词
D O I
10.1091/mbc.E08-01-0103
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
By screening yeast knockouts for their dependence upon the mitochondrial genome, we identified Mgr3p, a protein that associates with the i-AAA protease complex in the mitochondrial inner membrane. Mgr3p and Mgr1p, another i-AAA-interacting protein, form a subcomplex that bind to the i-AAA subunit Yme1p. We find that loss of Mgr3p, like the lack of Mgr1p, reduces proteolysis by Yme1p. Mgr3p and Mgr1p can bind substrate even in the absence of Yme1p, and both proteins are needed for maximal binding of an unfolded substrate by the i-AAA complex. We speculate that Mgr3p and Mgr1p function in an adaptor complex that targets substrates to the i-AAA protease for degradation.
引用
收藏
页码:5387 / 5397
页数:11
相关论文
共 60 条
[1]  
Adams A., 1997, METHODS YEAST GENETI
[2]   Evidence for a novel mitochondria-to-nucleus signalling pathway in respiring cells lacking i-AAA protease and the ABC-transporter Mdl1 [J].
Arnold, I ;
Wagner-Ecker, M ;
Ansorge, W ;
Langer, T .
GENE, 2006, 367 :74-88
[3]  
ATTARDI G, 1988, ANNU REV CELL BIOL, V4, P289, DOI 10.1146/annurev.cb.04.110188.001445
[4]   Characterization of peptides released from mitochondria -: Evidence for constant proteolysis and peptide efflux [J].
Augustin, S ;
Nolden, M ;
Müller, S ;
Hardt, O ;
Arnold, I ;
Langer, T .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (04) :2691-2699
[5]   Recycling of ESCRTs by the AAA-ATPase Vps4 is regulated by a conserved VSL region in Vta 1 [J].
Azmi, I ;
Davies, B ;
Dimaano, C ;
Payne, J ;
Eckert, D ;
Babst, M ;
Katzmann, DJ .
JOURNAL OF CELL BIOLOGY, 2006, 172 (05) :705-717
[6]   ATP-dependent proteases of bacteria: recognition logic and operating principles [J].
Baker, Tania A. ;
Sauer, Robert T. .
TRENDS IN BIOCHEMICAL SCIENCES, 2006, 31 (12) :647-653
[7]  
Brachmann CB, 1998, YEAST, V14, P115
[8]  
Chen XJ, 2000, INT REV CYTOL, V194, P197
[9]   α and β subunits of F1-ATPase are required for survival of petite mutants in Saccharomyces cerevisiae [J].
Chen, XJ ;
Clark-Walker, GD .
MOLECULAR AND GENERAL GENETICS, 1999, 262 (4-5) :898-908
[10]  
DAUM G, 1982, J BIOL CHEM, V257, P3028