Structure of Electron-Capture Dissociation Fragments from Charge-Tagged Peptides Probed by Tunable Infrared Multiple Photon Dissociation

被引：57

作者：

Frison, Gilles ^{[1
,2
]}

van der Rest, Guillaume ^{[1
,2
]}

Turecek, Frantisek ^{[3
]}

Besson, Thierry ^{[4
]}

Lemaire, Joel ^{[4
]}

Maitre, Philippe ^{[4
]}

Chamot-Rooke, Julia ^{[1
,2
]}

机构：

[1] Ecole Polytech, Dept Chem, Lab Mecanismes Reactionnels, F-91128 Palaiseau, France

[2] CNRS, F-91128 Palaiseau, France

[3] Univ Washington, Dept Chem, Seattle, WA USA

[4] Univ Paris 11, Chim Phys Lab, F-91405 Orsay, France

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2008年 / 130卷 / 45期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1021/ja805257v

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

In this study, we propose the first spectroscopic structural characterization of c-type ions produced by ECD of a peptide. The structure of c-type ions formed by electron capture dissociation and the overall mechanism leading to their formation are still a question of debate. Depending on the mechanism, c-type ions have been proposed to have either an enol-imine structure (-C(OH)=NH) or an amide one (-C(O)-NH2). Since these ions are isomeric, mass spectrometry only cannot discriminate between them, but infrared spectroscopy can bring experimental evidence and help determine which scheme is operative. Using the coupling between a tunable free electron laser and a FT-ICR mass spectrometer, we show that c-type ions have an amide structure, characterized by an IR signature of the C=O stretch at 1731 cm(-1). This result is particularly interesting from the perspective of the elucidation of the ECD mechanism.

引用

页码：14916 / +

页数：3

共 16 条

[1] Gas-phase structure of a π-allyl-palladium complex:: Efficient infrared Spectroscopy in a 7 T Fourier transform mass spectrometer [J].