Cryo-Immunoelectron Microscopy of Adherent Cells Improved by the Use of Electrospun Cell Culture Substrates

被引：6

作者：

Schmiedinger, Thomas ^{[1
]}

Vogel, Georg F. ^{[2
,3
]}

Eiter, Oliver ^{[1
]}

Pfaller, Kristian ^{[2
]}

Kaufmann, Walter A. ^{[4
]}

Floerl, Angelika ^{[2
]}

Gutleben, Karin ^{[2
]}

Schoenherr, Sabine ^{[4
]}

Witting, Barbara ^{[2
]}

Lechleitner, Thomas W. ^{[1
]}

Ebner, Hannes-L. ^{[2
,5
]}

Seppi, Thomas ^{[1
]}

Hess, Michael W. ^{[2
]}

机构：

[1] Med Univ Innsbruck, Dept Therapeut Radiol & Oncol, A-6020 Innsbruck, Austria

[2] Med Univ Innsbruck, Div Histol & Embryol, A-6020 Innsbruck, Austria

[3] Med Univ Innsbruck, Div Cell Biol, Bioctr, A-6020 Innsbruck, Austria

[4] Med Univ Innsbruck, Inst Pharmacol, A-6020 Innsbruck, Austria

[5] Med Univ Innsbruck, Dept Trauma Surg, A-6020 Innsbruck, Austria

来源：

TRAFFIC | 2013年 / 14卷 / 08期

关键词：

cell culture; cryofixation; cryosections; electrospinning; freeze-fracture replica labelling; freeze-; substitution; gelatin nanofibres; high-pressure freezing; immunogold; ULTRATHIN CRYOSECTIONS; FREEZE; TOMOGRAPHY; PROLIFERATION; LOCALIZATION; SUSPENSIONS; ATTACHMENT; MEMBRANES; EXIT;

D O I：

10.1111/tra.12080

中图分类号：

Q2 [细胞生物学];

学科分类号：

071013 [干细胞生物学];

摘要：

Electrospun nanofibres are an excellent cell culture substrate, enabling the fast and non-disruptive harvest and transfer of adherent cells for microscopical and biochemical analyses. Metabolic activity and cellular structures are maintained during the only half a minute-long harvest and transfer process. We show here that such samples can be optimally processed by means of cryofixation combined either with freeze-substitution, sample rehydration and cryosection-immunolabelling or with freeze-fracture replica-immunolabelling. Moreover, electrospun fibre substrates are equally suitable for complementary approaches, such as biochemistry, fluorescence microscopy and cytochemistry.

引用

页码：886 / 894

页数：9

共 49 条

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