Dynamic light scattering study of precrystallizing ribonuclease solutions

被引：27

作者：

Boyer, M ^{[1
]}

Roy, MO ^{[1
]}

Jullien, M ^{[1
]}

机构：

[1] UNIV MONTPELLIER 1,FAC PHARM,CTR BIOCHIM STRUCT,CNRS,UMR C9955,INSERM U414,F-34060 MONTPELLIER,FRANCE

来源：

JOURNAL OF CRYSTAL GROWTH | 1996年 / 167卷 / 1-2期

关键词：

D O I：

10.1016/0022-0248(96)00242-4

中图分类号：

O7 [晶体学];

学科分类号：

0702 ; 070205 ; 0703 ; 080501 ;

摘要：

The translational diffusion coefficient of bovine pancreatic ribonuclease A, a globular protein, has been measured by dynamic light scattering under various conditions related to the crystallization process. In all cases, a monomodal light scattering autocorrelation function was observed. The diffusion coefficient exhibited a linear dependence on protein concentration and an interaction parameter could be calculated. Attractive interactions were found to increase with salt concentration and to decrease with pH in the presence of salts. Finally, optimal crystallization conditions correspond to moderate attractive interactions.

引用

页码：212 / 220

页数：9

共 33 条

[1] BROWNIAN DIFFUSION OF PARTICLES WITH HYDRODYNAMIC INTERACTION [J].

BATCHELOR, GK .

JOURNAL OF FLUID MECHANICS, 1976, 74 (MAR9) :1-29

[2] CRYSTAL-STRUCTURE OF A FLUORESCENT DERIVATIVE OF RNASE-A [J].

BAUDETNESSLER, S ;

JULLIEN, M ;

CROSIO, MP ;

JANIN, J .

BIOCHEMISTRY, 1993, 32 (33) :8457-8464

[3]

Berne BJ, 1976, Dynamic Light Scattering

[4] SMALL-ANGLE NEUTRON-SCATTERING STUDY OF LYSOZYME SOLUTIONS [J].

BOUE, F ;

LEFAUCHEUX, F ;

ROBERT, MC ;

ROSENMAN, I .

JOURNAL OF CRYSTAL GROWTH, 1993, 133 (3-4) :246-254

[5]

CARTER CW, 1979, J BIOL CHEM, V254, P2219

[6] FLUORESCENCE STUDY OF PRECRYSTALLIZATION OF RIBONUCLEASE-A - EFFECT OF SALTS [J].

CROSIO, MP ;

JULLIEN, M .

JOURNAL OF CRYSTAL GROWTH, 1992, 122 (1-4) :66-70

[7] CRYSTAL PACKING IN 6 CRYSTAL FORMS OF PANCREATIC RIBONUCLEASE [J].

CROSIO, MP ;

JANIN, J ;

JULLIEN, M .

JOURNAL OF MOLECULAR BIOLOGY, 1992, 228 (01) :243-251

[8]

DUCRUIX A, UNPUB

[9] MOLECULAR-INTERACTIONS IN CRYSTALLIZING LYSOZYME SOLUTIONS STUDIED BY PHOTON-CORRELATION SPECTROSCOPY [J].

EBERSTEIN, W ;

GEORGALIS, Y ;

SAENGER, W .

JOURNAL OF CRYSTAL GROWTH, 1994, 143 (1-2) :71-78

[10]

EBERSTEIN W, 1993, EUR BIOPHYS J, V22, P259

← 1 2 3 4 →