Expression of vitamin D3 25-hydroxylase (CYP27) mRNA after induction by vitamin D3 or UVB radiation in keratinocytes of human skin equivalents -: a preliminary study

被引：56

作者：

Lehmann, B ^{[1
]}

Tiebel, O

Meurer, M

机构：

[1] Tech Univ Dresden, Carl Gustav Carus Med Sch, Dept Dermatol, D-01307 Dresden, Germany

[2] Tech Univ Dresden, Carl Gustav Carus Med Sch, Inst Clin Chem & Lab Med, D-01307 Dresden, Germany

来源：

ARCHIVES OF DERMATOLOGICAL RESEARCH | 1999年 / 291卷 / 09期

关键词：

keratinocyte; vitamin D-3; UVB radiation; vitamin D-3-25-hydroxylase; calcitriol;

D O I：

10.1007/s004030050445

中图分类号：

R75 [皮肤病学与性病学];

学科分类号：

100206 ;

摘要：

The presence of both la-hydroxylase activity and 25-hydroxylase activity is a biochemical prerequisite for the enzymatic generation of 1 alpha,25(OH)(2)D-3 from vitamin D-3 in keratinocytes. In contrast to 1 alpha-hydroxylase, however, activity of a 25-hydroxylase in he; ratinocytes has not previously been demonstrated. In this study, using RT-PCR we detected vitamin D-3 25-hydroxylase mRNA in human keratinocytes, The expression of vitamin D-3 25-hydroxylase mRNA in keratinocytes was identical to that of CYP27 expressed in mitochondria of human hepatoma HepG2 cells. CYP27 mRNA in keratinocytes is not constitutively expressed but is inducible both by vitamin D-3 (780 nM) and by UVB radiation (300 nm, 30 mJ/cm(2)).

引用

页码：507 / 510

页数：4

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